Rawle Daniel J, Nguyen Wilson, Dumenil Troy, Parry Rhys, Warrilow David, Tang Bing, Le Thuy T, Slonchak Andrii, Khromykh Alexander A, Lutzky Viviana P, Yan Kexin, Suhrbier Andreas
Inflammation Biology Group, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia.
School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, QLD 4072, Australia.
Pathogens. 2020 Oct 16;9(10):848. doi: 10.3390/pathogens9100848.
Getah virus (GETV) is a mosquito-transmitted alphavirus primarily associated with disease in horses and pigs in Asia. GETV was also reported to have been isolated from mosquitoes in Australia in 1961; however, retrieval and sequencing of the original isolates (N544 and N554), illustrated that these viruses were virtually identical to the 1955 GETV isolate from Malaysia. K-mer mining of the >40,000 terabases of sequence data in the Sequence Read Archive followed by BLASTn confirmation identified multiple GETV sequences in biosamples from Asia (often as contaminants), but not in biosamples from Australia. In contrast, sequence reads aligning to the Australian Ross River virus (RRV) were readily identified in Australian biosamples. To explore the serological relationship between GETV and other alphaviruses, an adult wild-type mouse model of GETV was established. High levels of cross-reactivity and cross-protection were evident for convalescent sera from mice infected with GETV or RRV, highlighting the difficulties associated with the interpretation of early serosurveys reporting GETV antibodies in Australian cattle and pigs. The evidence that GETV circulates in Australia is thus not compelling.
盖塔病毒（GETV）是一种由蚊子传播的甲病毒，主要与亚洲马匹和猪的疾病有关。1961年在澳大利亚也曾报道从蚊子中分离出盖塔病毒；然而，对原始分离株（N544和N554）的检索和测序表明，这些病毒实际上与1955年从马来西亚分离的盖塔病毒相同。在序列读取存档中对超过40000太字节的序列数据进行k-mer挖掘，随后通过BLASTn确认，在来自亚洲的生物样本中鉴定出多个盖塔病毒序列（通常作为污染物），但在来自澳大利亚的生物样本中未发现。相反，在澳大利亚的生物样本中很容易鉴定出与澳大利亚罗斯河病毒（RRV）比对的序列读数。为了探索盖塔病毒与其他甲病毒之间的血清学关系，建立了盖塔病毒成年野生型小鼠模型。感染盖塔病毒或罗斯河病毒的小鼠恢复期血清表现出高水平的交叉反应性和交叉保护作用，这突出了早期血清学调查在解释澳大利亚牛和猪中报告的盖塔病毒抗体时所面临的困难。因此，盖塔病毒在澳大利亚传播的证据并不确凿。
