Xing Chaonan, Jiang Jianfeng, Lu Zongji, Mi Shijiang, He Biao, Tu Changchun, Liu Xiufan, Gong Wenjie
College of Veterinary Medicine, Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou, China.
Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Changchun, China.
Transbound Emerg Dis. 2020 Sep;67(5):2249-2253. doi: 10.1111/tbed.13567. Epub 2020 Apr 19.
Getah virus (GETV) is a mosquito-borne virus that is widely distributed in Asian countries including China, in which the first case of equine GETV infection was reported in Guangdong province in August 2018. In this study, GETVs were detected in two classical swine fever virus-positive samples collected from swine herds in Foshan city, Guangdong province, 2018. Infection of porcine PK-15 cells produced rapid cytopathic effects (CPEs), including shrinking, rounding and detaching, and peak titre of 10 TCID /ml occurred at 24 hr post-infection. Electron microscopy and ultra-thin sectioning revealed spherical GETV particles of 70 nm diameter with an isometric interior and are found to be lining the outer membrane of infected cells. Whole-genome analysis showed that the two GETV isolates are identical to each other and cluster with Group III strains of GETV, sharing 96.1%-99.7% nucleotide sequence identity with all available reference strains. The most closely relative of the obtained GETV isolates was porcine strain HNJZ-S2 from Henan province (99.7%), with 98.6% sequence identity shared with equine GETV strain GZ201808 first identified in Guangdong province, indicating different sources for porcine and equine GETV infections in this region. No evidence of GETV infection was found in 497 archived porcine samples collected between 1990 and 2018 in Guangdong province.
盖塔病毒（GETV）是一种蚊媒病毒，广泛分布于包括中国在内的亚洲国家，2018年8月广东省报告了首例马感染盖塔病毒的病例。在本研究中，于2018年从广东省佛山市猪群采集的两份经典猪瘟病毒阳性样本中检测到盖塔病毒。猪PK - 15细胞感染后产生快速细胞病变效应（CPE），包括细胞收缩、变圆和脱落，感染后24小时出现最高滴度为10⁷TCID₅₀/ml。电子显微镜和超薄切片显示，盖塔病毒颗粒呈球形，直径70nm，内部呈等轴状，且发现其排列在受感染细胞的外膜上。全基因组分析表明，这两个盖塔病毒分离株彼此相同，与盖塔病毒Ⅲ组毒株聚类，与所有可用参考毒株的核苷酸序列同一性为96.1% - 99.7%。所获得的盖塔病毒分离株与河南省的猪毒株HNJZ - S2亲缘关系最近（99.7%），与2018年8月在广东省首次鉴定的马盖塔病毒毒株GZ201808的序列同一性为98.6%，表明该地区猪和马感染盖塔病毒的来源不同。在1990年至2018年期间于广东省采集的497份存档猪样本中未发现盖塔病毒感染的证据。
