Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.
Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan.
Curr Pharm Biotechnol. 2021;22(9):1254-1263. doi: 10.2174/1389201021666201020162158.
Nanomaterials have recently been identified for their potential benefits in the areas of medicine and pharmaceuticals. Among these nanomaterials, silver nanoparticles (Ag-NPs) have been widely utilized in the fields of diagnostics, antimicrobials, and catalysis.
To investigate the potential utility of Citrobacter freundii in the synthesis of silver Nanoparticles (Ag-NPs), and to determine the antimicrobial activities of the Ag-NPs produced.
Aqueous Ag+ ions were reduced when exposed to C. freundii extract and sunlight, leading to the formation of Ag-NPs. Qualitative microanalysis for the synthesized Ag-NPs was done using UVvis spectrometry, Energy Dispersive X-ray analysis (EDX), and scanning and transmission electron microscopy. The hydrodynamic size and stability of the particles were detected using Dynamic Light Scattering (DLS) analysis. The Ag-NPs' anti-planktonic and anti-biofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa, which are two important skin and wound pathogens, were investigated. The cytotoxicity on human dermal fibroblast cell line was also determined.
Ag-NPs were spherical with a size range between 15 to 30 nm. Furthermore, Ag-NPs displayed potent bactericidal activities against both S. aureus and P. aeruginosa and showed noticeable anti-biofilm activity against S. aureus biofilms. Ag-NPs induced minor cytotoxic effects on human cells as indicated by a reduction in cell viability, a disruption of plasma membrane integrity, and apoptosis induction.
Ag-NPs generated in this study might be a future potential alternative to be used as antimicrobial agents in pharmaceutical applications for wound and skin related infections.
纳米材料因其在医学和制药领域的潜在应用而受到关注。在这些纳米材料中，银纳米粒子（Ag-NPs）已广泛应用于诊断、抗菌和催化领域。
研究弗氏柠檬酸杆菌在合成银纳米粒子（Ag-NPs）中的潜在应用，并确定所合成的 Ag-NPs 的抗菌活性。
当暴露于弗氏柠檬酸杆菌提取物和阳光下时，Ag+离子被还原，形成 Ag-NPs。采用紫外可见分光光度法、能谱分析（EDX）、扫描和透射电子显微镜对所合成的 Ag-NPs 进行定性微观分析。使用动态光散射（DLS）分析检测颗粒的水动力粒径和稳定性。研究了 Ag-NPs 对金黄色葡萄球菌和铜绿假单胞菌（两种重要的皮肤和伤口病原体）的浮游生物和生物膜的抗药性。还测定了对人皮肤成纤维细胞系的细胞毒性。
Ag-NPs 呈球形，粒径在 15 至 30nm 之间。此外，Ag-NPs 对金黄色葡萄球菌和铜绿假单胞菌具有很强的杀菌活性，对金黄色葡萄球菌生物膜也具有明显的抗生物膜活性。Ag-NPs 对人细胞的细胞毒性较小，表现为细胞活力降低、质膜完整性破坏和诱导细胞凋亡。
本研究中生成的 Ag-NPs 可能成为未来在制药领域用于治疗与伤口和皮肤相关的感染的潜在抗菌剂。
