麻风病患者对人源及纯化犰狳源麻风分枝杆菌、卡介苗和结核菌素纯蛋白衍生物的淋巴细胞反应。
Lymphocyte response of leprosy patients to human-derived and purified armadillo-derived Mycobacterium leprae, BCG and PPD.
作者信息
Smelt A H, Liew F Y, Rees R J
出版信息
Clin Exp Immunol. 1978 Nov;34(2):164-9.
The lymphocyte transformation test was applied to compare in vitro lymphocyte responses of tuberculoid (high resistant) and lepromatous (low resistant) leprosy patients to purified Mycobacterium leprae derived from experimentally infected armadillos and crude M. leprae derived from man, as well as to bacille Calmette-Guérin (BCG) and purified protein derivative (PPD). It was found that the purification procedure using enzymic digestion did not affect the immunogenicity of armadillo-derived M. leprae as compared with the crude human-derived preparation, although 2.5-5-fold higher doses of the purified organisms were required to elicitate equivalent lymphocyte responses. The result indicated the suitability of purified armadillo-derived M. leprae as the standard antigen for lymphocytes transformation tests in leprosy. The cross-reactivity studies show a close relationship between PPD and BCG, but not between M. leprae and PPD or BCG.
应用淋巴细胞转化试验比较结核样型（高抗性）和瘤型（低抗性）麻风患者的体外淋巴细胞对源自实验感染犰狳的纯化麻风分枝杆菌、源自人类的粗制麻风分枝杆菌、卡介苗（BCG）和纯化蛋白衍生物（PPD）的反应。结果发现，与粗制的源自人类的制剂相比，使用酶消化的纯化程序并未影响源自犰狳的麻风分枝杆菌的免疫原性，尽管需要高2.5至5倍剂量的纯化菌才能引发同等的淋巴细胞反应。结果表明，纯化的源自犰狳的麻风分枝杆菌适合作为麻风病淋巴细胞转化试验的标准抗原。交叉反应性研究表明PPD与BCG之间关系密切，但麻风分枝杆菌与PPD或BCG之间没有密切关系。
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