Nivedita Lukram, Prakash Venkatachalam, Mitthra Suresh, Pearlin Mary Newbegin Selvakumar Gold, Venkatesh Alagarsamy, Subbiya Arunajatesan
Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India.
J Conserv Dent. 2019 Sep-Oct;22(5):483-489. doi: 10.4103/JCD.JCD_195_20. Epub 2020 Aug 4.
The objective is to evaluate the effect of collagen stabilizing agents-chitosan and proanthocyanidin (PA) on the shear bond strength to dentin and microleakage of resin composite at enamel and cemental walls.
Thirty premolars were decoronated 2 mm above cemento-enamel junction and restored with composite resin. Teeth were then randomly divided into three groups: Group I - Control, Group II - Pre-treatment with chitosan, and Group III - Pre-treatment with PA. Samples were then subjected to thermocycling for 500 cycles at 5°C and 55°C with the dwell time of 30 s and transfer time of 5-10 s. Then, the samples were subjected to shear bond strength evaluation on Universal testing machine. Shear load was applied until failure occurred. The load to failure was recorded individually and statistical analysis was done. Microleakage was determined by methylene blue dye penetration method and subjected to stereomicroscopic evaluation. Statistical analysis was carried out using Mann-Whitney test and Chi-square test.
Group II specimens produced the highest median shear bond strength and group I showed the least. In addition, Group I, Group II, and Group III showed no statistically significant difference in microleakage.
Application of Chitosan and PA improved the shear bond strength to dentin as compared to the control. However, no significant difference in shear bond strength and microleakage was found between them.
评估胶原蛋白稳定剂——壳聚糖和原花青素（PA）对树脂复合材料与牙本质的剪切粘结强度以及在釉质壁和牙骨质壁处微渗漏的影响。
30颗前磨牙在牙骨质 - 釉质界上方2mm处去冠，并用复合树脂修复。然后将牙齿随机分为三组：第一组 - 对照组，第二组 - 用壳聚糖预处理，第三组 - 用PA预处理。接着将样本在5°C和55°C下进行500次热循环，保压时间为30秒，转移时间为5 - 10秒。之后，在万能试验机上对样本进行剪切粘结强度评估。施加剪切载荷直至发生破坏。分别记录破坏载荷并进行统计分析。通过亚甲蓝染料渗透法测定微渗漏，并进行体视显微镜评估。使用曼 - 惠特尼检验和卡方检验进行统计分析。
第二组样本产生的中位剪切粘结强度最高，第一组最低。此外，第一组、第二组和第三组在微渗漏方面无统计学显著差异。
与对照组相比，壳聚糖和PA的应用提高了与牙本质的剪切粘结强度。然而，它们在剪切粘结强度和微渗漏方面未发现显著差异。
