Minnich L L, Shehab Z M, Ray C G
Department of Pathology, Arizona Health Sciences Center, Tucson 85724.
Diagn Microbiol Infect Dis. 1987 Jun;7(2):137-41. doi: 10.1016/0732-8893(87)90031-9.
A fluorescein isothiocyanate-conjugated pool of monoclonal antibodies (MoAb) to respiratory syncytial virus (RSV) was prospectively evaluated for its utility as a direct, 1-hr test for the diagnosis of RSV infection. Direct nasopharyngeal swab smears collected from 109 infants and children with acute respiratory illnesses were studied and compared with results obtained by indirect immunofluorescence using bovine polyclonal anti-RSV antibody on eluted cells derived from pooled nasopharyngeal and throat swab specimens (a 2.5-3 hr procedure), and culture. The MoAb-direct smear method was at least 86%-89% sensitive and 95%-100% specific compared with either of the other procedures. Additional prospective evaluations, as well as retrospective studies on a selected bank of slides stored from the preceding year, established that this MoAb could also be used with confidence in testing where direct smears are not employed.
对一组用异硫氰酸荧光素偶联的抗呼吸道合胞病毒（RSV）单克隆抗体（MoAb）进行了前瞻性评估，以确定其作为诊断RSV感染的直接1小时检测方法的实用性。研究了从109例患有急性呼吸道疾病的婴幼儿中采集的直接鼻咽拭子涂片，并与使用牛抗RSV多克隆抗体对汇集的鼻咽和咽喉拭子标本洗脱细胞进行间接免疫荧光检测（2.5 - 3小时的操作流程）以及培养所获得的结果进行比较。与其他任何一种检测方法相比，MoAb直接涂片法的敏感性至少为86% - 89%，特异性为95% - 100%。额外的前瞻性评估以及对前一年存储的一组选定玻片的回顾性研究证实，这种MoAb在不采用直接涂片的检测中也可放心使用。
