Olsen M A, Shuck K M, Sambol A R, Bohnert V A, Henery M L
Department of Medical Microbiology, Creighton University School of Medicine, Omaha, NE 68178.
Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):325-9. doi: 10.1016/0732-8893(93)90084-k.
The Kallestad Pathfinder enzyme immunoassay (EIA) for the rapid detection of respiratory syncytial virus (RSV) antigen was compared with virus culture and direct fluorescent antibody (DFA) to determine the reliability of the EIA. During two consecutive winter respiratory seasons, 270 nasopharyngeal wash specimens were tested. RSV was detected in culture by the presence of cytopathic effect and/or an indirect immunofluorescence assay. The sensitivity of the Pathfinder EIA in comparison with isolation in tube culture was 72% (73 of 101) and the specificity was 99% (167 of 169). During the second year of the evaluation period, DFA was performed on all specimens. The sensitivity of the DFA compared with isolation in tube culture was 94%. This study indicates that the Pathfinder EIA is a very specific test for diagnosis of RSV infections, but lacks sensitivity in comparison with tube culture or direct immunofluorescence.
将用于快速检测呼吸道合胞病毒（RSV）抗原的卡莱斯塔德探路者酶免疫测定法（EIA）与病毒培养法和直接荧光抗体法（DFA）进行比较，以确定该酶免疫测定法的可靠性。在连续两个冬季呼吸道疾病流行季节，对270份鼻咽冲洗液标本进行了检测。通过细胞病变效应的存在和/或间接免疫荧光测定法在培养物中检测到RSV。与试管培养法相比，探路者酶免疫测定法的灵敏度为72%（101份标本中有73份检测到RSV），特异性为99%（169份标本中有167份）。在评估期的第二年，对所有标本进行了直接荧光抗体检测。与试管培养法相比，直接荧光抗体检测的灵敏度为94%。本研究表明，探路者酶免疫测定法是诊断RSV感染的一种非常特异的检测方法，但与试管培养法或直接免疫荧光法相比，缺乏灵敏度。
