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环状 RNA RNF13/miRNA-1224-5p 轴调控促进急性髓系白血病恶性演变。

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia.

机构信息

Department of Hematology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China.

出版信息

Biomed Res Int. 2020 Oct 6;2020:5654380. doi: 10.1155/2020/5654380. eCollection 2020.

DOI:10.1155/2020/5654380
PMID:33083473
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7557902/
Abstract

OBJECTIVE

To study the biological function of circular RNA RNF13 (circRNF13) in acute myeloid leukemia (AML) and its relationship with prognosis.

METHODS

We constructed stable AML cell lines with downregulated expression of circRNF13, and then, we explored the effect of downregulation of circRNF13 expression on the proliferation, migration, and invasion through qRT-PCR, MTT curve, colony formation, transwell migration and invasion experiment, cell cycle, apoptosis, Caspase 3/7 assay, and other experiments. We also studied the expression of C-myc and Tenascin-C by qRT-PCR to explore the role of circRNF13.

RESULTS

When the expression of circRNF13 was downregulated, the proliferation rate of AML cells decreased significantly, the cell cycle was blocked to G1 phase, and apoptosis rate increased significantly. C-myc related to cell proliferation decreased significantly at RNA level. Furthermore, when the expression of circRNF13 was downregulated, the migration and invasion ability of AML cells was significantly reduced, and the expression of Tenascin-C related to migration and invasion also decreased significantly. The luciferase reporter assay system confirmed that miRNA-1224-5p was the direct target of circRNF13.

CONCLUSION

CircRNF13 inhibited the proliferation, migration, and invasion of AML cells by regulating the expression of miRNA-1224-5p. This study provides some clues for the diagnosis and treatment of AML.

摘要

目的

研究环状 RNA RNF13（circRNF13）在急性髓系白血病（AML）中的生物学功能及其与预后的关系。

方法

构建circRNF13 表达下调的稳定 AML 细胞系，然后通过 qRT-PCR、MTT 曲线、集落形成、transwell 迁移和侵袭实验、细胞周期、凋亡、Caspase 3/7 测定等实验，探讨下调 circRNF13 表达对细胞增殖、迁移和侵袭的影响。还通过 qRT-PCR 研究了 C-myc 和 Tenascin-C 的表达，以探讨 circRNF13 的作用。

结果

下调 circRNF13 的表达后，AML 细胞的增殖率显著下降，细胞周期阻滞于 G1 期，凋亡率显著增加。与细胞增殖相关的 C-myc 在 RNA 水平上显著下降。此外，下调 circRNF13 的表达后，AML 细胞的迁移和侵袭能力显著降低，与迁移和侵袭相关的 Tenascin-C 的表达也显著降低。荧光素酶报告基因检测系统证实，miRNA-1224-5p 是 circRNF13 的直接靶标。

结论

circRNF13 通过调节 miRNA-1224-5p 的表达抑制 AML 细胞的增殖、迁移和侵袭。本研究为 AML 的诊断和治疗提供了一些线索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/bf6597ae1fed/BMRI2020-5654380.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/719786b7f9e8/BMRI2020-5654380.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/80474cfadc43/BMRI2020-5654380.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/c8f12128882e/BMRI2020-5654380.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/4decb0c93cc5/BMRI2020-5654380.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/662a4afe618a/BMRI2020-5654380.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/bf6597ae1fed/BMRI2020-5654380.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/719786b7f9e8/BMRI2020-5654380.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/80474cfadc43/BMRI2020-5654380.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/c8f12128882e/BMRI2020-5654380.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/4decb0c93cc5/BMRI2020-5654380.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/662a4afe618a/BMRI2020-5654380.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b855/7557902/bf6597ae1fed/BMRI2020-5654380.006.jpg

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