Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, China.
Department of Hematology, Heze Municipal Hospital, Heze 274000, China.
Biomed Res Int. 2020 Oct 6;2020:7023168. doi: 10.1155/2020/7023168. eCollection 2020.
Acute myeloid leukemia (AML) is a heterogeneous hematological malignancy, whose immunological mechanisms are still partially uncovered. Regulatory B cells (Bregs) and CD4+ regulatory T cells (Tregs) are subgroups of immunoregulatory cells involved in modulating autoimmunity, inflammation, and transplantation reactions. Herein, by studying the number and function of Breg and Treg cell subsets in patients with AML, we explored their potential role in the pathogenesis of AML. Newly diagnosed AML patients, AML patients in complete remission, and healthy controls were enrolled. Flow cytometry was used to detect percentages of Bregs and Tregs. ELISA was conducted to detect IL-10 and TGF- in plasma. The mRNA levels of IL-10 and Foxp3 were measured with RT-qPCR. The relationship of Bregs and Tregs with the clinicopathological parameters was analyzed. There was a significant reduction in the frequencies of Bregs and an increase of Tregs in newly diagnosed AML patients compared with healthy controls. Meanwhile, patients in complete remission exhibited levels of Bregs and Tregs comparable to healthy controls. Furthermore, compared with healthy controls and AML patients in complete remission, newly diagnosed AML patients had increased plasma IL-10 but reduced TGF-. IL-10 and Foxp3 mRNA levels were upregulated in the newly diagnosed AML patients. However, there were no significant differences in IL-10 and Foxp3 mRNA levels between patients in complete remission and healthy controls. Bregs and Tregs have abnormal distribution in AML patients, suggesting that they might play an important role in regulating immune responses in AML.
急性髓系白血病（AML）是一种异质性血液恶性肿瘤，其免疫机制仍部分未被揭示。调节性 B 细胞（Bregs）和 CD4+调节性 T 细胞（Tregs）是参与调节自身免疫、炎症和移植反应的免疫调节细胞亚群。在此，通过研究 AML 患者 Breg 和 Treg 细胞亚群的数量和功能，我们探讨了它们在 AML 发病机制中的潜在作用。纳入新诊断的 AML 患者、AML 患者完全缓解和健康对照者。流式细胞术用于检测 Breg 和 Treg 细胞亚群的百分比。ELISA 用于检测血浆中的 IL-10 和 TGF-。使用 RT-qPCR 测量 IL-10 和 Foxp3 的 mRNA 水平。分析 Bregs 和 Tregs 与临床病理参数的关系。与健康对照组相比，新诊断的 AML 患者 Bregs 的频率显著降低，Tregs 的频率增加。同时，完全缓解的患者的 Bregs 和 Tregs 水平与健康对照组相当。此外，与健康对照组和完全缓解的 AML 患者相比，新诊断的 AML 患者的血浆 IL-10 增加，但 TGF-减少。新诊断的 AML 患者的 IL-10 和 Foxp3 mRNA 水平上调。然而，完全缓解的患者和健康对照组之间的 IL-10 和 Foxp3 mRNA 水平没有显著差异。AML 患者中 Bregs 和 Tregs 分布异常，提示它们可能在调节 AML 中的免疫反应中发挥重要作用。
