Department of Hematology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China.
Hematology and Oncology Unit, Department of Geriatrics, Qilu Hospital of Shandong University, Jinan, China.
BMC Cancer. 2022 May 21;22(1):568. doi: 10.1186/s12885-022-09532-1.
Cadherin-23 (CDH23) plays an important role in intercellular adhesion and is involved in the progression of several types of cancer. However, the biological functions and effect of CDH23 expression on the prognosis of patients with acute myeloid leukemia (AML) are unexplored. Herein, we aim to characterize the role and molecular functions of CDH23 in AML.
We downloaded the transcriptomic profiles and clinical data from the Cancer Genome Atlas and Beat AML trial. The expression level of CDH23 was assessed using Gene Expression Profiling Interactive Analysis (GEPIA). Kaplan-Meier survival analysis was used to assess prognostic value of CDH23. Correlation and biological function analyses were performed using LinkedOmics and GeneMANIA. Relationship of CDH23 with immune infiltration level was determined using Tumor Immune Estimation Resource (TIMER).
We found that the CDH23 expression was aberrantly upregulated in patients with AML and could be used as an independent risk factor of overall survival using Cox multivariate analysis. Notably, we observed a negative correlation between CDH23 expression and immune cell infiltration abundance by calculating the immune and stromal scores. In addition, functional enrichment analysis established that CDH23 plays a crucial role in tumor immunity.
Our findings indicate that upregulated CDH23 expression corresponds to decreased overall survival of patients with AML. CDH23 may be involved in mediating tumor immune environment, and this highlights the potential of CDH23 as a therapeutic target in AML.
钙黏蛋白 23（CDH23）在细胞间黏附中发挥重要作用，并参与多种类型癌症的进展。然而，CDH23 表达对急性髓系白血病（AML）患者预后的生物学功能和影响尚未被探索。在此，我们旨在描述 CDH23 在 AML 中的作用和分子功能。
我们从癌症基因组图谱（TCGA）和 Beat AML 试验中下载转录组谱和临床数据。使用基因表达谱交互式分析（GEPIA）评估 CDH23 的表达水平。Kaplan-Meier 生存分析用于评估 CDH23 的预后价值。使用 LinkedOmics 和 GeneMANIA 进行相关性和生物学功能分析。使用肿瘤免疫估计资源（TIMER）确定 CDH23 与免疫浸润水平的关系。
我们发现 AML 患者的 CDH23 表达异常上调，并且可以通过 Cox 多变量分析将其用作总生存的独立危险因素。值得注意的是，通过计算免疫和基质评分，我们观察到 CDH23 表达与免疫细胞浸润丰度之间存在负相关。此外，功能富集分析表明 CDH23 在肿瘤免疫中起着至关重要的作用。
我们的研究结果表明，CDH23 表达上调与 AML 患者总生存率降低相关。CDH23 可能参与介导肿瘤免疫微环境，这突显了 CDH23 作为 AML 治疗靶点的潜力。
