慢性乙型肝炎（CHB）或慢性丙型肝炎（CHC）患者中，CD5+CD19+CD1d高表达IL-10+B调节细胞、CD4+Foxp3+调节性T细胞（Tregs）、CD4+CXCR5+Foxp3+滤泡调节性T（TFR）细胞数量增加。

Increased numbers of CD5+CD19+CD1dhighIL-10+ Bregs, CD4+Foxp3+ Tregs, CD4+CXCR5+Foxp3+ follicular regulatory T (TFR) cells in CHB or CHC patients.

作者信息

Wang Li, Qiu Jinpeng, Yu Lei, Hu Xiaoli, Zhao Pingwei, Jiang Yanfang

机构信息

Key Laboratory of Zoonosis Research, Ministry of Education, the First Hospital of Jilin University, Changchun 130021, China.

出版信息

J Transl Med. 2014 Sep 9;12:251. doi: 10.1186/s12967-014-0251-9.

BACKGROUND

IL-10+ regulatory B (Bregs), CD4+Foxp3+ regulatory T (Tregs), and CD4+CXCR5+Foxp3+ follicular regulatory T (TFR) cells regulate the progression of infection disease. This study aimed at examining how those cells associated with the development of chronic hepatitis B (CHB) and chronic hepatitis C (CHC) in a Chinese population.

METHODS

The numbers of circulating IL-10+ Bregs, Tregs and TFR cells in 31 CHC, 58 CHB patients and 22 healthy controls (HC) were examined by flow cytometry. The potential association of those cells with clinical measures was analyzed.

RESULTS

The numbers of CD5+CD19+CD1dhighIL-10+ Bregs, Tregs and TFR cells and the levels of serum IL-10, IFN-γ and IL-2 in the CHB, and IL-10 and IFN-γ in the CHC patients were significantly higher than that in the HC (p<0.05). Furthermore, the numbers of circulating IL-10+ Bregs and the levels of serum IL-10, but not other cytokines tested were positively correlated with the levels of serum HBV DNA and ALT in the HBeAg- CHB patients as well as HCV RNA and ALT in CHC patients. Additionally, the numbers of circulating TFR cells were positively correlated with the levels of serum HBV DNA and ALT in the CHB patients as well as HCV RNA and ALT in the CHC patients.

CONCLUSIONS

Increased numbers of circulating IL-10+ Bregs and TFR cells are associated with poor virus eradication and liver injury in CHB and CHC patients. Furthermore, the levels of serum IL-10 is associated with the hepatic flares.

背景

白细胞介素10（IL-10）+调节性B细胞（Bregs）、CD4+叉头框蛋白3（Foxp3）+调节性T细胞（Tregs）和CD4+CXC趋化因子受体5（CXCR5）+Foxp3+滤泡调节性T细胞（TFR）调节感染性疾病的进展。本研究旨在探讨这些细胞与中国人群慢性乙型肝炎（CHB）和慢性丙型肝炎（CHC）发生发展的关系。

方法

采用流式细胞术检测31例CHC患者、58例CHB患者和22例健康对照（HC）外周血中循环IL-10+Bregs、Tregs和TFR细胞的数量，并分析这些细胞与临床指标的潜在关联。

结果

CHB患者中CD5+CD19+CD1dhighIL-10+Bregs、Tregs和TFR细胞数量以及血清IL-10、干扰素-γ（IFN-γ）和白细胞介素2（IL-2）水平，CHC患者中IL-10和IFN-γ水平均显著高于HC（p<0.05）。此外，HBeAg阴性CHB患者外周血循环IL-10+Bregs数量及血清IL-10水平（而非其他检测的细胞因子）与血清乙肝病毒脱氧核糖核酸（HBV DNA）及丙氨酸氨基转移酶（ALT）水平呈正相关，CHC患者外周血循环IL-10+Bregs数量及血清IL-10水平与血清丙肝病毒核糖核酸（HCV RNA）及ALT水平呈正相关。此外，CHB患者外周血循环TFR细胞数量与血清HBV DNA及ALT水平呈正相关，CHC患者外周血循环TFR细胞数量与血清HCV RNA及ALT水平呈正相关。