Menis Candela Florencia, Giordano Walter Fabián, Quiroga Patricia Liliana, Escobar Franco Matías, Mañas Fernando, Roma Dardo Andrés, Larrauri Mariana, Comini Laura Raquel, Soria Elio Andrés, Sabini María Carola
Universidad Nacional de Río Cuarto, Departamento de Microbiología e Inmunología, Área Virología, Ruta 36 Km 601, Río Cuarto, Córdoba, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, INBIAS, CONICET, Fac. Cs. Exactas Fco Qcas y Nat, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba, Argentina.
Heliyon. 2020 Oct 8;6(10):e05119. doi: 10.1016/j.heliyon.2020.e05119. eCollection 2020 Oct.
L. (Leguminosae) is distributed in tropical and subtropical areas. Peanut has high nutritional and commercial value. Scientific research showed that peanut has biological properties such as anticancer, antioxidant, antiinflammatory. However, it is necessary to know if consumption of peanut, either as food or as a phytopharmaceutical implies a health risk. The aim was to evaluate cytotoxicity and genotoxicity of ethanolic extracts from . Also, chemical characterization of these extracts was performed. Cytotoxicity was evaluated by MTT and Neutral Red Uptake (NRU) assays on Vero cells. Genotoxicity was studied by Micronuclei and comet assays on Balb/C mice. Qualitative and quantitative chemical analysis of extracts were performed. Results showed that extracts have low cytotoxicity. Tegument ethanolic extract (TEE) and Seed ethanolic extract (SEE) were not genotoxic. The treatments with TEE at 250 mg/kg and SEE at 2000 mg/kg revealed (highest concentrations evaluated) some toxicity on blood marrow cells of mice. Chemical characterization indicated that TEE had 74.33 ± 1.10 mg GAE/g of dried extract and SEE had 15.05 ± 0.06 mg GAE/g of dried extract of total phenolic content. Also, proanthocyanidins (O.D. at 550 nm 1.39 ± 0.15) and caffeic acid (2.46%) were identified in TEE. While, linoleic acid (58.84%) oleic acid (11.31%) and palmitic acid (8.37%) were major compounds of SEE. In conclusion, peanut consumption is safe at concentrations recommended for healthy uses, such as nutrition, and phytomedicine.
豆科植物分布于热带和亚热带地区。花生具有很高的营养和商业价值。科学研究表明，花生具有抗癌、抗氧化、抗炎等生物学特性。然而，有必要了解食用花生，无论是作为食物还是作为植物药，是否意味着存在健康风险。目的是评估[具体植物名称]乙醇提取物的细胞毒性和遗传毒性。此外，还对这些提取物进行了化学表征。通过MTT和中性红摄取（NRU）试验在Vero细胞上评估细胞毒性。通过微核试验和彗星试验在Balb/C小鼠上研究遗传毒性。对提取物进行了定性和定量化学分析。结果表明，提取物具有低细胞毒性。种皮乙醇提取物（TEE）和种子乙醇提取物（SEE）没有遗传毒性。用250mg/kg的TEE和2000mg/kg的SEE处理（评估的最高浓度）显示对小鼠骨髓细胞有一定毒性。化学表征表明，TEE的总酚含量为74.33±1.10mg GAE/g干提取物，SEE为15.05±0.06mg GAE/g干提取物。此外，在TEE中鉴定出原花青素（550nm处吸光度为1.39±0.15）和咖啡酸（2.46%）。而亚油酸（58.84%）、油酸（11.31%）和棕榈酸（8.37%）是SEE的主要成分。总之，按照健康用途推荐的浓度食用花生是安全的，如营养和植物医学方面。 （注：原文中“L. (Leguminosae)”未明确具体植物名称，翻译时保留原文形式）
