Fu Yu, Liu Xiaobei, Wang Qian, Liu Huan, Cheng Yumeng, Li Hongmei, Zhang Yong, Chen Julian
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Ministry of Agricultural and Rural Affairs-Centre for Agriculture and Bioscience International (MARA-CABI) Joint Laboratory for Bio-Safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2023 Mar 28;14:1104275. doi: 10.3389/fpls.2023.1104275. eCollection 2023.
The grain aphid is a serious pest of wheat that causes severe economic damage by sucking phloem sap and transmitting plant viruses. Here, two putative salivary effector homologs from (Sm10 and SmC002) were selected based on sequence similarity to other characterized aphid candidate effectors. These effectors were then delivered into wheat cells separately the type III secretion system of to elucidate their functions in the regulation of plant defenses and host fitness. The results showed that the delivery of either Sm10 or SmC002 into wheat plants significantly suppressed callose deposition and affected the transcript levels of callose synthase genes. The expression levels of salicylic acid (SA)-associated defense genes were upregulated significantly in wheat leaves carrying either Sm10 or SmC002. Moreover, LC-MS/MS analysis revealed that wheat SA levels significantly increased after the delivery of the two effectors. The results of aphid bioassays conducted on the wheat plants carrying Sm10 or SmC002 showed significant increases in the survival and fecundity of . This study demonstrated that the Sm10 and SmC002 salivary effectors of enhanced host plant susceptibility and benefited performance by regulating wheat defense signaling pathways.
麦长管蚜是小麦的一种严重害虫，通过吸食韧皮部汁液和传播植物病毒造成严重的经济损失。在此，基于与其他已鉴定的蚜虫候选效应子的序列相似性，从[蚜虫名称未给出]中选择了两个假定的唾液效应子同源物（Sm10和SmC002）。然后，通过[细菌名称未给出]的III型分泌系统将这些效应子分别导入小麦细胞，以阐明它们在调节植物防御和宿主适应性方面的功能。结果表明，将Sm10或SmC002导入小麦植株显著抑制了胼胝质沉积，并影响了胼胝质合成酶基因的转录水平。在携带Sm10或SmC002的小麦叶片中，水杨酸（SA）相关防御基因的表达水平显著上调。此外，液相色谱-串联质谱（LC-MS/MS）分析表明，导入这两种效应子后，小麦中的SA水平显著增加。对携带Sm10或SmC002的小麦植株进行的蚜虫生物测定结果表明，[蚜虫名称未给出]的存活率和繁殖力显著提高。本研究表明，[蚜虫名称未给出]的Sm10和SmC002唾液效应子通过调节小麦防御信号通路增强了宿主植物的易感性，并有利于[蚜虫名称未给出]的生长性能。
