FOCAS Research Institute, Technological University Dublin, 13 Camden Row, D08CKP1 Dublin, Ireland.
Unidad de Hepatología Experimental, Health Research Institute Hospital La Fe, Av. Fernando Abril Martorell, 46026 Valencia, Spain.
Anal Chem. 2020 Nov 3;92(21):14542-14549. doi: 10.1021/acs.analchem.0c02735. Epub 2020 Oct 21.
The estimation of steatosis in a liver graft is mandatory prior to liver transplantation, as the risk of graft failure increases with the level of infiltrated fat. However, the assessment of liver steatosis before transplantation is typically based on a qualitative or semiquantitative characterization by visual inspection and palpation and histological analysis. Thus, there is an unmet need for transplantation surgeons to have access to a diagnostic tool enabling an in situ fast classification of grafts prior to extraction. In this study, we have assessed an attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopic method compatible with the requirements of an operation room for the evaluation of the lipid contents in human livers. A set of 20 human liver biopsies obtained from organs intended for transplantation were analyzed by expert pathologists, ATR-FTIR spectroscopy, lipid biochemical analysis, and UPLC-ESI(+/-)TOFMS for lipidomic profiling. Comparative analysis of multisource data showed strong correlations between ATR-FTIR, clinical, and lipidomic information. Results show that ATR-FTIR captures a global picture of the lipid composition of the liver, along with information for the quantification of the triradylglycerol content in liver biopsies. Although the methodology performance needs to be further validated, results support the applicability of ATR-FTIR for the in situ determination of the grade of liver steatosis at the operation room as a fast, quantitative method, as an alternative to the qualitative and subjective pathological examination.
在进行肝移植之前，必须对供体肝组织中的脂肪浸润程度进行评估，因为脂肪浸润程度越高，移植物发生衰竭的风险就越大。然而，在移植前对肝脂肪变性的评估通常基于视觉检查和触诊以及组织学分析的定性或半定量特征。因此，移植外科医生迫切需要一种诊断工具，以便在提取供体前对其进行原位快速分类。在这项研究中，我们评估了一种与手术室要求兼容的衰减全反射-傅里叶变换红外（ATR-FTIR）光谱方法，用于评估人类肝脏中的脂质含量。一组 20 例来自拟用于移植的器官的肝活检标本由专家病理学家进行分析，同时进行 ATR-FTIR 光谱分析、脂质生化分析以及 UPLC-ESI(+/-)TOFMS 脂质组学分析。多源数据的比较分析表明，ATR-FTIR 与临床和脂质组学信息之间存在很强的相关性。结果表明，ATR-FTIR 可以捕获肝脏脂质组成的全貌，同时还可以定量分析肝活检中三酰甘油的含量。尽管该方法的性能需要进一步验证，但结果支持 ATR-FTIR 作为一种快速、定量的方法，替代定性和主观的病理检查，用于在手术室现场确定肝脂肪变性的程度。
