Kobrin M S, Asa S L, Samsoondar J, Kudlow J E
Department of Clinical Biochemistry, University of Toronto, Ontario, Canada.
Endocrinology. 1987 Oct;121(4):1412-6. doi: 10.1210/endo-121-4-1412.
A growth factor secreted by bovine calf anterior pituitary cells in culture was purified, and its N-terminal amino acid sequence was determined. This sequence shows near-identity with human and rat alpha-transforming growth factor (alpha TGF). With the use of an anti-alpha TGF monoclonal antibody generated against a C-terminal rat alpha TGF synthetic peptide, alpha TGF-like material was localized by immunohistochemical techniques in the cytoplasm of normal bovine adenohypophysial cells. The antibody staining was immunospecific because it could be completely inhibited by saturating concentrations of the synthetic peptide to which it was raised. There was no immunoreactivity in cells of the intermediate and posterior lobes. Some of the cells containing alpha TGF immunoreactivity also contained PRL; alpha TGF immunoreactivity was not demonstrated in cells containing ACTH, TSH, FSH, and LH. This is the first report documenting the secretion of alpha TGF by nonneoplastic adult cells and the presence of alpha TGF immunoreactivity in the corresponding normal adult tissue.
对培养的牛犊前脑垂体细胞分泌的一种生长因子进行了纯化，并测定了其N端氨基酸序列。该序列与人及大鼠的α-转化生长因子（αTGF）几乎完全相同。利用针对大鼠αTGF C端合成肽产生的抗αTGF单克隆抗体，通过免疫组织化学技术在正常牛腺垂体细胞的细胞质中定位了αTGF样物质。抗体染色具有免疫特异性，因为它可以被产生该抗体的合成肽的饱和浓度完全抑制。中间叶和后叶细胞没有免疫反应性。一些含有αTGF免疫反应性的细胞也含有催乳素；含有促肾上腺皮质激素、促甲状腺激素、促卵泡激素和促黄体生成素的细胞中未显示αTGF免疫反应性。这是第一份记录非肿瘤性成年细胞分泌αTGF以及相应正常成年组织中存在αTGF免疫反应性的报告。
