Department of Biochemistry and Molecular Genetics, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.
Department of Medicine, Division of Haematology, University of Colorado Denver - Anschutz Medical Campus, Aurora, CO, United States of America.
Blood Transfus. 2021 May;19(3):216-223. doi: 10.2450/2020.0145-20. Epub 2020 Oct 14.
The impact of donor biology on blood component storability is increasingly appreciated as a determinant of the storage lesion and post-transfusion performances. Platelet metabolism is affected by age and it is critical to platelet responses to activating stimuli in an age-dependent manner. Sex has been previously highlighted as a contributing factor to the platelet proteomics lesion. However, little is known about the impact of donor sex and age on stored platelet metabolism and post-transfusion capacity to circulate.
Apheresis platelets were donated via apheresis by 21 healthy volunteers (12 males and 9 females; ages 20 to 59). Metabolomics analyses were performed at day 0 and after 5 days of storage at 22+2 °C, along with autologous post-transfusion recovery and survival studies with Cr and In.
Sex and age significantly impacted platelet metabolism at baseline and upon storage. Platelets from older, male donors were characterised by higher levels of Krebs cycle metabolites, pentose phosphate pathway intermediates and byproducts, deaminated purines and long chain fatty acids. These metabolites ranked amongst the top significant correlates to post-transfusion recoveries. Glutathione homeostasis and sphingosine 1-phosphate were the top positive correlates to long term survival, which was lower in platelets from older, male donors - without reaching statistical significance.
In this study we report that donor sex and age have a significant impact on platelet metabolism. Novel metabolic correlates to platelet post-transfusion performances (24 h recovery and long-term survival) were identified through high-resolution, stable isotope-labeled internal standard-assisted metabolomics approach.
供者生物学对血液成分储存稳定性的影响越来越被认为是储存损伤和输血后性能的决定因素。血小板代谢受年龄影响，年龄对血小板对激活刺激的反应有重要影响。性别先前已被强调为血小板蛋白质组学损伤的一个影响因素。然而，关于供者性别和年龄对储存血小板代谢和输血后循环能力的影响知之甚少。
通过 21 名健康志愿者（12 名男性和 9 名女性；年龄 20 至 59 岁）进行机采血小板采集。在 22+2°C 下储存 5 天后，进行代谢组学分析，并进行自体输血后的恢复和 Cr 和 In 的生存研究。
性别和年龄在基线和储存时显著影响血小板代谢。来自年龄较大的男性供者的血小板表现出更高水平的三羧酸循环代谢物、戊糖磷酸途径中间产物和副产物、脱氨嘌呤和长链脂肪酸。这些代谢物是与输血后恢复最显著相关的因素之一。谷胱甘肽稳态和 1-磷酸鞘氨醇是与长期生存的正相关因素，而来自年龄较大的男性供者的血小板的长期生存能力较低，但没有达到统计学意义。
在这项研究中，我们报告供者性别和年龄对血小板代谢有显著影响。通过高分辨率、稳定同位素标记内部标准辅助代谢组学方法，确定了与血小板输血后性能（24 小时恢复和长期生存）相关的新型代谢物。
