Department of Plant breeding, Swedish University of Agricultural Sciences, 23053 Alnarp, Sweden.
Genes (Basel). 2020 Oct 19;11(10):1223. doi: 10.3390/genes11101223.
Domestication of a new crop requires identification and improvement of desirable characteristics Field cress is being domesticated as a new oilseed crop, particularly for northern temperate regions.. In the present study, an F mapping population and its F progenies were used to identify quantitative trait loci (QTLs) for plant height (PH), number of stems per plant (NS), stem growth orientation (SO), flowering habit (FH), earliness (ER), seed yield per plant (SY), pod shattering resistance (SHR), and perenniality (PE). A highly significant correlation ( < 0.001) was observed between several pairs of characteristics, including SY and ER (negative) or ER and PE (positive). The inclusive composite interval mapping approach was used for QTL mapping using 2330 single nucleotide polymorphism (SNP) markers mapped across the eight field cress linkage groups. Nine QTLs were identified with NS, PH, SO, and PE having 3, 3, 2, and 1 QTLs, explaining 21.3%, 29.5%, 3.8%, and 7.2% of the phenotypic variation, respectively. Candidate genes behind three of the QTLs and favorable marker alleles for different classes of each characteristic were identified. Following their validation through further study, the identified QTLs and associated favorable marker alleles can be used in marker-aided breeding to speed up the domestication of field cress.
驯化一种新作物需要确定和改进所需的特性。荠 正在被驯化成为一种新的油料作物，特别是在北方温带地区。本研究利用 F1 作图群体及其 F2 后代，鉴定了株高（PH）、单株茎数（NS）、茎生长方向（SO）、开花习性（FH）、早熟性（ER）、单株种子产量（SY）、荚果抗裂性（SHR）和多年生性（PE）的数量性状基因座（QTLs）。几个特性之间存在显著的相关性（<0.001），包括 SY 和 ER（负相关）或 ER 和 PE（正相关）。使用 2330 个单核苷酸多态性（SNP）标记，对包含 8 个荠 连锁群的 F1 作图群体及其 F2 后代进行了全基因组关联分析，共鉴定到 9 个 QTLs，其中 NS、PH、SO 和 PE 分别有 3、3、2 和 1 个 QTL，解释了 21.3%、29.5%、3.8%和 7.2%的表型变异。鉴定到的 3 个 QTL 背后的候选基因，以及每个特性不同类别的有利标记等位基因。通过进一步的研究验证后，这些鉴定到的 QTLs 和相关的有利标记等位基因可以用于标记辅助选择，以加快荠的驯化进程。
