Kurihara H, Uchida K
Shionogi Research Laboratories, Osaka, Japan.
Histochemistry. 1987;87(3):223-7. doi: 10.1007/BF00492413.
We investigated the distribution of microtubules and microfilaments in some exocrine and endocrine cells in rats. Microtubules were stained by applying an immunofluorescent technique using antibodies against beta-tubulin, while microfilaments were stained with rhodamine-phalloidin, which binds selectively to polymerized actin filaments. In the cytoplasm of some exocrine cells (pancreatic acinar cells and ventral prostatic epithelial cells), the microtubules were distributed longitudinally from the apical region to the basal region, but no microtubules were found in the nuclear region. In exocrine cells, most of the microfilaments were localized beneath the apical plasma membrane. In some endocrine cells (those of the adenohypophysis and the islets of Langerhans), the microtubules exhibited a radial or reticular distribution in the cytoplasm, and intense fluorescence was observed in the perinuclear region. The immunofluorescence produced by the antibodies against beta-tubulin was more intense in endocrine cells than in exocrine cells. The microfilaments observed in the endocrine cells studied were homogenously distributed beneath the plasma membrane. Dot-like rhodamine-phalloidin staining was often observed in the cytoplasm of both the exocrine and endocrine cells. The present study clearly demonstrated marked differences in the distribution of cytoskeletal elements in exocrine and endocrine cells, and these may reflect differences in the secretory direction of such cells as well as in epithelial-cell polarity.
我们研究了大鼠一些外分泌细胞和内分泌细胞中微管和微丝的分布情况。微管用抗β-微管蛋白抗体通过免疫荧光技术染色，而微丝用罗丹明-鬼笔环肽染色，它能选择性地结合聚合的肌动蛋白丝。在一些外分泌细胞（胰腺腺泡细胞和前列腺腹侧上皮细胞）的细胞质中，微管从顶端区域纵向分布到基部区域，但在核区域未发现微管。在外分泌细胞中，大多数微丝位于顶端质膜下方。在一些内分泌细胞（腺垂体细胞和胰岛细胞）中，微管在细胞质中呈放射状或网状分布，在核周区域观察到强烈荧光。抗β-微管蛋白抗体产生的免疫荧光在内分泌细胞中比在外分泌细胞中更强。在所研究的内分泌细胞中观察到的微丝均匀分布在质膜下方。在外分泌细胞和内分泌细胞的细胞质中经常观察到点状的罗丹明-鬼笔环肽染色。本研究清楚地表明外分泌细胞和内分泌细胞中细胞骨架成分的分布存在明显差异，这些差异可能反映了这些细胞分泌方向以及上皮细胞极性的差异。
