酚类化合物传统提取技术与替代提取技术的最新进展及比较
Recent advances and comparisons of conventional and alternative extraction techniques of phenolic compounds.
作者信息
Osorio-Tobón J Felipe
机构信息
Faculty of Health Sciences, University Institution Colegio Mayor de Antioquia (COLMAYOR), Carrera 78 # 65-46, Medellín, 050036 Antioquia Colombia.
出版信息
J Food Sci Technol. 2020 Dec;57(12):4299-4315. doi: 10.1007/s13197-020-04433-2. Epub 2020 May 28.
Phenolic compounds are a group of secondary metabolites produced by plants under stressful conditions. Phenolic compounds play an important role in the prevention and treatment of certain illnesses and are exploited by the food and pharmaceutical industries. Conventional methods are commonly used as models to compare the efficiencies of alternative extraction methods. Among alternative extraction processes, microwave-assisted extraction (MAE), pressurized liquid extraction (PLE), supercritical fluid extraction (SFE) and ultrasonic-assisted extraction (UAE) are the most studied. These methods produce extracts rich in phenolic compounds using moderate temperatures, short extraction times, and solvents generally recognized as safe. The combination of extraction time and temperature plays a critical role in the stability of the compounds. Solvents of higher polarity enhance the extraction of phenolic compounds. The use of the ethanol-water mixture for MAE, PLE, and UAE is recommended. MAE and UAE involve shorter extraction times than do PLE and SFE. SFE requires a low average temperature (40 °C). MAE produces the highest total phenolic content [227.63 mg GAE/g dry basis (d.b.)], followed by PLE (173.65 mg GAE/g d.b.), UAE (92.99 mg GAE/g d.b.) and SFE (37 mg GAE/g d.b.). Extraction yields and recovery rates of the phenolic compounds can be enhanced by combining and integrating extraction methods.
酚类化合物是植物在胁迫条件下产生的一类次生代谢产物。酚类化合物在某些疾病的预防和治疗中发挥着重要作用,并且被食品和制药行业所利用。传统方法通常被用作模型来比较替代提取方法的效率。在替代提取工艺中,微波辅助提取(MAE)、加压液体提取(PLE)、超临界流体提取(SFE)和超声辅助提取(UAE)是研究最多的。这些方法使用适中的温度、较短的提取时间以及通常被认为安全的溶剂来生产富含酚类化合物的提取物。提取时间和温度的组合对化合物的稳定性起着关键作用。较高极性的溶剂能增强酚类化合物的提取。建议将乙醇 - 水混合物用于MAE、PLE和UAE。MAE和UAE的提取时间比PLE和SFE短。SFE需要较低的平均温度(40°C)。MAE产生的总酚含量最高[227.63毫克没食子酸当量/克干基(d.b.)],其次是PLE(173.65毫克没食子酸当量/克d.b.)、UAE(92.99毫克没食子酸当量/克d.b.)和SFE(37毫克没食子酸当量/克d.b.)。通过组合和整合提取方法可以提高酚类化合物的提取率和回收率。
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