Department of Analytical Chemistry, University of Granada, E-18071 Granada, Spain.
Molecules. 2010 Dec 3;15(12):8813-26. doi: 10.3390/molecules15128813.
This paper reviews the phenolic-compound-extraction systems used to analyse fruit and vegetable samples over the last 10 years. Phenolic compounds are naturally occurring antioxidants, usually found in fruits and vegetables. Sample preparation for analytical studies is necessary to determine the polyphenolic composition in these matrices. The most widely used extraction system is liquid-liquid extraction (LLE), which is an inexpensive method since it involves the use of organic solvents, but it requires long extraction times, giving rise to possible extract degradation. Likewise, solid-phase extraction (SPE) can be used in liquid samples. Modern techniques, which have been replacing conventional ones, include: supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). These alternative techniques reduce considerably the use of solvents and accelerate the extraction process.
本文综述了过去 10 年中用于分析水果和蔬菜样品的酚类化合物提取系统。酚类化合物是天然存在的抗氧化剂，通常存在于水果和蔬菜中。为了确定这些基质中的多酚组成，需要对分析研究中的样品进行预处理。最广泛使用的提取系统是液-液萃取（LLE），这是一种廉价的方法，因为它涉及使用有机溶剂，但它需要较长的提取时间，可能导致提取物降解。同样，固相萃取（SPE）也可用于液体样品。现代技术，已经取代了传统技术，包括：超临界流体萃取（SFE）、加压液体萃取（PLE）、微波辅助萃取（MAE）和超声辅助萃取（UAE）。这些替代技术大大减少了溶剂的使用并加速了提取过程。
