Sahoo Swastideepa, Sanghamitra Priyadarsini, Nanda Nibedita, Pawar Swapnil, Pandit Elssa, Bastia Ramakrushna, Muduli Kumuda Chandra, Pradhan Sharat Kumar
Department of Seed Technology, College of Agriculture, OUAT, Bhubaneswar, 751003 Odisha India.
Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, Odisha 753006 India.
Physiol Mol Biol Plants. 2020 Oct;26(10):1989-2003. doi: 10.1007/s12298-020-00879-y. Epub 2020 Sep 14.
Eighteen physio-biochemical traits influencing seed vigour were studied for their association with molecular markers using a mini core set constituted from 120 germplasm lines. High genetic variation was detected in the parameters namely chlrophyll a, Chlrophyll b, total chlorophyll, carotenoids, total anthocyanin content, gamma-oryzanols, total phenolics content, superoxide dismutase, catalase, guaicol peroxidase, total soluble sugar, total protein, seed vigour index -I and seed vigour index -II. Strong positive correlation of seed vigour index II was observed with amylose content, total anthocyanin content, catalase, total phenolic content and total flavonoid content while a negative association was observed for gamma-oryzanol content. High gene diversity (0.7169) and informative markers value (0.6789) were estimated from the investigation. Three genetic structure groups were observed in the panel population and genotypes were grouped in the subpopulations based on the seed vigour trait. Differences in the fixation indices of the three sub populations indicated existence of linkage disequilibrium in the studied panel population. Association of the traits namely total flavonoids, superoxide dismutase, catalase, chlorophyll a, Chlorophyll b, total chlorophyll, carotenoids, starch, amylose, total anthocyanin, gamma-oryzanol, total phenolics with the molecular markers were detected by Generalized Linear Model and Mixed Linear Model showing > 0.10 R value. Association of the trait, total flavonoids with marker RM7364 located on chromosome 8 reported in earlier study was validated in this investigation. The validated markers and the novel markers detected showing higher R value will be useful for improvement of seed vigour in rice.
利用由120份种质系构成的微型核心种质库，研究了18个影响种子活力的生理生化性状与分子标记的关联。在所测定的参数中，即叶绿素a、叶绿素b、总叶绿素、类胡萝卜素、总花青素含量、γ-谷维素、总酚含量、超氧化物歧化酶、过氧化氢酶、愈创木酚过氧化物酶、总可溶性糖、总蛋白、种子活力指数-I和种子活力指数-II，检测到了较高的遗传变异。种子活力指数II与直链淀粉含量、总花青素含量、过氧化氢酶、总酚含量和总黄酮含量呈强正相关，而与γ-谷维素含量呈负相关。通过调查估计出较高的基因多样性（0.7169）和信息标记值（0.6789）。在该群体中观察到三个遗传结构组，并根据种子活力性状将基因型分组到亚群中。三个亚群的固定指数差异表明，在所研究的群体中存在连锁不平衡。通过广义线性模型和混合线性模型检测到总黄酮、超氧化物歧化酶、过氧化氢酶、叶绿素a、叶绿素b、总叶绿素、类胡萝卜素、淀粉、直链淀粉、总花青素、γ-谷维素、总酚等性状与分子标记的关联，R值>0.10。在本研究中验证了先前研究报道的位于第8染色体上的标记RM7364与总黄酮性状的关联。所验证的标记和检测到的显示较高R值的新标记将有助于提高水稻种子活力。
