Aggarwal Sakshi, Kumar Ashish, Jain Muskan, Sudan Jebi, Singh Kapil, Kumari Sumita, Mustafiz Ananda
Plant Molecular Biology Laboratory, Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, 110021 India.
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu, 180009 India.
Physiol Mol Biol Plants. 2020 Oct;26(10):2019-2033. doi: 10.1007/s12298-020-00881-4. Epub 2020 Sep 21.
Intracellular signaling is a critical determinant of the normal growth and development of plants. Signaling peptides, also known as peptide hormones, along with classical phytohormones, are the significant players of plant intracellular signaling. C-terminally encoded peptide (CEP), a 15-amino acid post-translationally peptide identified in Arabidopsis, plays a pivotal role in lateral root formation, nodulation, and act as long-distance root to shoot signaling molecule in N-starvation conditions. Expression of CEP gene members in Arabidopsis is perturbed by nitrogen starvation; however, not much is known regarding their role in other abiotic stress conditions. To gain a comprehensive insight into CEP biology, we identified CEP genes across diverse plant genera (, , , and ) using bioinformatics tools. In silico promoter analysis revealed that CEP gene promoters show an abundance of abiotic stress-responsive elements suggesting a possible role of CEPs in abiotic stress signaling. Spatial and temporal expression patterns of CEP via RNA seq and microarray revealed that various CEP genes are transcriptionally regulated in response to abiotic stresses. Validation of rice CEP genes expression by qRT-PCR showed that , , , and were highly upregulated in response to different abiotic stress conditions. Our findings suggest these CEP genes might be important mediators of the abiotic stress response and warrant further overexpression/knockout studies to delineate their precise role in abiotic stress response.
细胞内信号传导是植物正常生长和发育的关键决定因素。信号肽，也被称为肽激素，与经典植物激素一起，是植物细胞内信号传导的重要参与者。C末端编码肽（CEP）是在拟南芥中鉴定出的一种15个氨基酸的翻译后肽，在侧根形成、结瘤中起关键作用，并在氮饥饿条件下作为从根到地上部的长距离信号分子。拟南芥中CEP基因成员的表达受到氮饥饿的干扰；然而，关于它们在其他非生物胁迫条件下的作用知之甚少。为了全面深入了解CEP生物学，我们使用生物信息学工具在不同植物属（、、、和）中鉴定了CEP基因。电子启动子分析表明，CEP基因启动子含有大量非生物胁迫响应元件，表明CEP在非生物胁迫信号传导中可能发挥作用。通过RNA测序和微阵列分析CEP的时空表达模式，发现各种CEP基因在转录水平上受非生物胁迫调控。通过qRT-PCR验证水稻CEP基因的表达，结果表明，、、、在不同非生物胁迫条件下均高度上调。我们的研究结果表明，这些CEP基因可能是非生物胁迫响应的重要调节因子，值得进一步进行过表达/敲除研究以阐明它们在非生物胁迫响应中的精确作用。
