Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry of Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
School of Basic Medicine, Hubei University of Medicine, Shiyan 442000, China.
Plant Cell Physiol. 2023 Feb 16;64(1):19-26. doi: 10.1093/pcp/pcac171.
C-TERMINALLY ENCODED PEPTIDEs (CEPs) are post-translationally modified peptides that play essential roles in root and shoot development, nitrogen absorption, nodule formation and stress resilience. However, it has proven challenging to determine biological activities of CEPs because of difficulties in obtaining loss-of-function mutants for these small genes. To overcome this challenge, we thus assembled a collection of easily detectable large fragment deletion mutants of Arabidopsis CEP genes through the clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9-engineered genome editing. This collection was then evaluated for the usability by functionally analyzing the Arabidopsis growth and development with a focus on the root. Most cep mutants displayed developmental defects in primary and lateral roots showing an increased primary root length and an enhanced lateral root number, demonstrating that the genetic resource provides a useful tool for further investigations into the roles of CEPs.
C-末端编码肽（CEPs）是经过翻译后修饰的肽，在根和茎的发育、氮吸收、根瘤形成和应激弹性中发挥着重要作用。然而，由于这些小基因的功能丧失突变体难以获得，因此确定 CEPs 的生物学活性具有一定的挑战性。为了克服这一挑战，我们通过簇状规则间隔短回文重复序列/CRISPR 相关蛋白 9 工程基因组编辑，组装了一套易于检测的拟南芥 CEP 基因的大片段缺失突变体库。然后，通过重点研究根，从功能上分析拟南芥的生长和发育来评估该突变体库的可用性。大多数 cep 突变体在主根和侧根的发育过程中表现出缺陷，表现为主根长度增加和侧根数量增加，这表明该遗传资源为进一步研究 CEPs 的作用提供了有用的工具。
