Wen Xin, Chen Yuan-Hua, Li Rong, Ge Ming-Hai, Yin Sheng-Wu, Wu Jing-Jing, Huang Jia-Hao, Liu Hui, Wang Ping-Zhou, Gross Einav, Wu Zheng-Xing
Key Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Department of Biochemistry & Molecular Biology, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
iScience. 2020 Sep 19;23(10):101588. doi: 10.1016/j.isci.2020.101588. eCollection 2020 Oct 23.
Animals' ability to sense environmental cues and to integrate this information to control fecundity is vital for continuing the species lineage. In this study, we observed that the sensory neurons Amphid neuron (ASHs and ADLs) differentially regulate egg-laying behavior in under varied environmental conditions via distinct neuronal circuits. Under standard culture conditions, ASHs tonically release a small amount of glutamate and inhibit Hermaphrodite specific motor neuron (HSN) activities and egg laying via a highly sensitive Glutamate receptor (GLR)-5 receptor. In contrast, under Cu stimulation, ASHs and ADLs may release a large amount of glutamate and inhibit Amphid interneuron (AIA) interneurons via low-sensitivity Glutamate-gated chloride channel (GLC)-3 receptor, thus removing the inhibitory roles of AIAs on HSN activity and egg laying. However, directly measuring the amount of glutamate released by sensory neurons under different conditions and assaying the binding kinetics of receptors with the neurotransmitter are still required to support this study directly.
动物感知环境线索并整合这些信息以控制繁殖力的能力对于物种延续至关重要。在本研究中，我们观察到感觉神经元腹侧神经节神经元（ASHs和ADLs）在不同环境条件下通过不同的神经回路差异调节产卵行为。在标准培养条件下，ASHs持续释放少量谷氨酸，并通过高度敏感的谷氨酸受体（GLR）-5受体抑制雌雄同体特异性运动神经元（HSN）的活动和产卵。相比之下，在铜刺激下，ASHs和ADLs可能释放大量谷氨酸，并通过低敏感性谷氨酸门控氯离子通道（GLC）-3受体抑制腹侧神经节中间神经元（AIA）中间神经元，从而消除AIA对HSN活动和产卵的抑制作用。然而，仍需要直接测量不同条件下感觉神经元释放的谷氨酸量，并分析受体与神经递质的结合动力学，以直接支持本研究。
