Liu Hui, Wu Jing-Jing, Li Rong, Wang Ping-Zhou, Huang Jia-Hao, Xu Yu, Zhao Jia-Lu, Wu Piao-Ping, Li Si-Jia, Wu Zheng-Xing
Key Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Front Mol Neurosci. 2023 Mar 15;16:1101628. doi: 10.3389/fnmol.2023.1101628. eCollection 2023.
Sensations, especially nociception, are tightly controlled and regulated by the central and peripheral nervous systems. Osmotic sensation and related physiological and behavioral reactions are essential for animal well-being and survival. In this study, we find that interaction between secondary nociceptive ADL and primary nociceptive ASH neurons upregulates avoidance of the mild and medium hyperosmolality of 0.41 and 0.88 Osm but does not affect avoidance of high osmolality of 1.37 and 2.29 Osm. The interaction between ASH and ADL is actualized through a negative feedback circuit consisting of ASH, ADL, and RIM interneurons. In this circuit, hyperosmolality-sensitive ADL augments the ASH hyperosmotic response and animal hyperosmotic avoidance; RIM inhibits ADL and is excited by ASH; thus, ASH exciting RIM reduces ADL augmenting ASH. The neuronal signal integration modality in the circuit is disexcitation. In addition, ASH promotes hyperosmotic avoidance through ASH/RIC/AIY feedforward circuit. Finally, we find that in addition to ASH and ADL, multiple sensory neurons are involved in hyperosmotic sensation and avoidance behavior.
感觉，尤其是伤害感受，受到中枢神经系统和外周神经系统的严格控制和调节。渗透感觉以及相关的生理和行为反应对于动物的健康和生存至关重要。在本研究中，我们发现次级伤害感受性ADL神经元与初级伤害感受性ASH神经元之间的相互作用上调了对0.41和0.88 Osm的轻度和中度高渗的回避反应，但不影响对1.37和2.29 Osm的高渗的回避反应。ASH和ADL之间的相互作用是通过由ASH、ADL和RIM中间神经元组成的负反馈回路实现的。在这个回路中，高渗敏感的ADL增强了ASH的高渗反应和动物的高渗回避反应；RIM抑制ADL并被ASH兴奋；因此，ASH兴奋RIM会减少ADL对ASH的增强作用。该回路中的神经元信号整合模式为去兴奋。此外，ASH通过ASH/RIC/AIY前馈回路促进高渗回避反应。最后，我们发现除了ASH和ADL之外，多个感觉神经元也参与高渗感觉和回避行为。
