Amos W B, Amos L A, Linck R W
Department of Zoology, Cambridge, UK.
J Cell Sci Suppl. 1986;5:55-68. doi: 10.1242/jcs.1986.supplement_5.4.
Chemically resistant 2-3 nm filaments with a high alpha-helical content, isolated from sea-urchin sperm flagellar doublet microtubules, consist of proteins that have been named tektins. Polyclonal affinity-purified antibodies to tektins labelled sperm tails all along their lengths, as shown by indirect immunofluorescence microscopy, provided the specimens were not too well fixed. Results obtained for unfixed specimens studied by immunoelectron microscopy suggested the tektins are normally masked by tubulin. A monoclonal anti-tektin antibody labelled bare tektin filaments at longitudinal intervals of approximately 48 nm, which fits in well with the 96 nm longitudinal repeat of axonemes. We discuss a possible scheme for the regular interaction of tubulin monomers with an alpha-helical coiled coil.
从海胆精子鞭毛双联体微管中分离出的具有高α-螺旋含量、抗化学腐蚀的2 - 3纳米细丝，由被命名为tektin的蛋白质组成。用间接免疫荧光显微镜观察发现，针对tektin的多克隆亲和纯化抗体可沿精子尾部全长标记，前提是标本固定得不过于充分。免疫电子显微镜对未固定标本的研究结果表明，tektin通常被微管蛋白掩盖。一种单克隆抗tektin抗体以约48纳米的纵向间隔标记裸露的tektin细丝，这与轴丝96纳米的纵向重复很好地吻合。我们讨论了微管蛋白单体与α-螺旋卷曲螺旋正常相互作用的一种可能机制。
