Wang Ting, Fu Jing, Liu Xin-Qiang, Zhang Qian
Department of Orthodontics, Affiliated Hospital of Qingdao University. Qingdao 266500, Shandong Province, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2020 Aug;29(4):350-354.
To investigate the expression and significance of chemokines CCL21, E-selectins and heat shock protein 90 (Hsp90) in periodontal tissues of rats with experimental periodontitis.
Forty 10-week-old male Wistar rats were significantly randomly divided into 4 groups with 10 rats in each group. Periodontitis models were established in groups A, B and C, and the rest were 10 blank control groups. Rats in group A, B and C were sacrificed at 4, 8 and 12 weeks after basic periodontal treatment, and the periodontal tissues of the first and second molars were taken for CCL21, E-selectins and Hsp90 protein expression detection. SPSS 25.0 software package was used to analyze the data.
The levels of periodontal attachment in group A, B and C were higher than those in the control group(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in periodontal tissues increased first and then decreased(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 mRNA and protein expression in group B and C were significantly higher than those in group A(P<0.05). The levels of periodontal attachment, CCL21, E-selectins, Hsp90 and relative protein expression in periodontal tissues of group C were significantly lower than those of group B(P<0.05).
The expression of CCL21, E-selectins and Hsp90 is up-regulated in periodontitis tissues. With local periodontal treatment, the expression level of CCL21, E-selectins and Hsp90 gradually decreases.
探讨趋化因子CCL21、E-选择素和热休克蛋白90（Hsp90）在实验性牙周炎大鼠牙周组织中的表达及意义。
将40只10周龄雄性Wistar大鼠随机分为4组，每组10只。A、B、C组建立牙周炎模型，其余为10只空白对照组。A、B、C组大鼠在基础牙周治疗后4、8、12周处死，取第一、二磨牙牙周组织进行CCL21、E-选择素和Hsp90蛋白表达检测。采用SPSS 25.0软件包进行数据分析。
A、B、C组牙周附着水平高于对照组（P<0.05）。牙周组织中牙周附着、CCL21、E-选择素、Hsp90 mRNA及蛋白表达水平先升高后降低（P<0.05）。B、C组牙周附着、CCL21、E-选择素、Hsp90 mRNA及蛋白表达水平显著高于A组（P<0.05）。C组牙周组织中牙周附着、CCL21、E-选择素、Hsp90及相关蛋白表达水平显著低于B组（P<0.05）。
牙周炎组织中CCL21、E-选择素和Hsp90表达上调。随着局部牙周治疗，CCL21、E-选择素和Hsp90表达水平逐渐降低。
