Rybacek L, D'Andrea M, Tarnowski S J
Process Development and Improvement Department, Interferon Sciences, Inc., New Brunswick, NJ 08901.
J Chromatogr. 1987 Jun 26;397:355-64. doi: 10.1016/s0021-9673(01)85019-6.
A rapid dual-column chromatographic assay for determining recombinant leukocyte interferon alpha-2 in complex mixtures is described. The assay relies on the use of a high-performance monoclonal antibody affinity column connected in tandem with a reversed-phase column. The high specificity and selectivity of these columns permits the quantitation of subcomponent species, such as interferon oligomers that may be present in assay samples. The assay has a limit of sensitivity equal to 1 microgram/g over a range of 1-20 micrograms/g. The precision of the assay was estimated to be about 5%.
描述了一种用于测定复杂混合物中重组白细胞干扰素α-2的快速双柱色谱分析方法。该分析方法依赖于串联连接的高效单克隆抗体亲和柱和反相柱的使用。这些柱的高特异性和选择性允许对分析样品中可能存在的亚组分种类进行定量，例如干扰素寡聚体。该分析方法在1 - 20微克/克的范围内灵敏度极限等于1微克/克。该分析方法的精密度估计约为5%。
