Roy S K, McGregor W C, Orichowskyj S T
J Chromatogr. 1985 Jun 26;327:189-92. doi: 10.1016/s0021-9673(01)81648-4.
A rapid assay for recombinant leukocyte A interferon has been developed that includes a small immunosorbent column (Amicon glass column ca. 10 X 6 mm; i.e., ca. 0.5 ml), containing monoclonal antibody, immobilized on Nugel-polyhydroxy phase silica, 500 A, 200-400 mesh (Diagnostic Specialties, Metuchen, NJ, U.S.A.). The column has been automated so that the operator need only inject sample (0.25 ml) every 18 min (or one can use an automatic sample injector) and initiate the program cycle of the microprocessor. A hard-copy result from an integrator is available in less than 20 min. Routine analyses were performed at a flow-rate of 4 ml/min in the concentration range 0.02-0.3 mg/ml. Reproducibility of the assay was checked by assaying the same crude extract seven times in succession. Standard deviation was 3.96% and correlation coefficient was 0.9996. The advantages of this technique include rapid analysis time and relative simplicity, compared to the enzyme immunoassay.
已开发出一种用于重组白细胞 A 干扰素的快速检测方法，该方法包括一个小型免疫吸附柱（Amicon 玻璃柱，约 10×6 毫米，即约 0.5 毫升），其中含有固定在 Nugel - 多羟基相硅胶（500 Å，200 - 400 目，美国新泽西州梅图申市诊断专业公司）上的单克隆抗体。该柱已实现自动化，操作人员只需每 18 分钟进样一次（0.25 毫升）（也可使用自动进样器）并启动微处理器的程序循环。积分仪在不到 20 分钟内即可提供硬拷贝结果。常规分析在流速为 4 毫升/分钟、浓度范围为 0.02 - 0.3 毫克/毫升的条件下进行。通过连续七次检测同一份粗提物来检查该检测方法的重现性。标准偏差为 3.96%，相关系数为 0.9996。与酶免疫测定法相比，该技术的优点包括分析时间短和相对简单。
