Kostrov S V, Borukhov S I, Eremashvili M R, Novikov A A, Skvortsova M A
Antibiot Med Biotekhnol. 1987 Apr;32(4):243-7.
Rapid and sensitive methods for immunochemical estimation of bacterially-made human leukocytic interferons were developed. A large number of strains producing such interferons was analyzed with these methods and optimal ones capable of synthesizing up to 10(10), 10(8), 10(9) and 10(9) IU/l of interferons alpha A, alpha N, alpha F and alpha I1 respectively were selected. The proteins were isolated in homogenous state and their main physico-chemical parameters were characterized. Interferon oligomers were detected. Their formation was partially due to intermolecular disulphide exchange. The effect on interferon alpha A of retinal (vitamin A) and its derivatives efficiently interacting with this protein was studied.
开发了用于免疫化学估算细菌产生的人白细胞干扰素的快速灵敏方法。用这些方法分析了大量产生此类干扰素的菌株，并筛选出了分别能够合成高达10¹⁰、10⁸、10⁹和10⁹ IU/L的αA、αN、αF和αI1干扰素的最佳菌株。以均一状态分离出蛋白质并对其主要物理化学参数进行了表征。检测到了干扰素寡聚体。其形成部分归因于分子间二硫键交换。研究了视黄醛（维生素A）及其与该蛋白质有效相互作用的衍生物对αA干扰素的影响。
