Department of Analytical Chemistry, Faculty of Pharmacy, Egyptian Russian University, Cairo, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Nasr City, Cairo, 11751, Egypt.
J Fluoresc. 2021 Jan;31(1):97-106. doi: 10.1007/s10895-020-02639-3. Epub 2020 Oct 22.
This study is the first to develop and optimize a method for the simultaneous determination of chlorthalidone (CLT) and telmisartan (TEL) in, human plasma samples as well as in their newly released pharmaceutical tablet form, (Telmikind-CT 40®). The method is based on measuring fluorescence intensity, employing synchronous fluorescence mode coupled to third-order derivative signal processing, 0.5% w/v cetyl trimethyl ammonium bromide was used as cationic surfactant to enhance the fluorescence signal intensity and improve method sensitivity. The third-order derivative synchronous spectra of CLT and TEL are well separated with two zero-crossing points which allowed for the determination of CLT and TEL at 362 nm and 351 nm, respectively. Different experimental parameters were carefully investigated and optimized, calibration curves were constructed over concentration ranges of 20-1200 ng.mL and 5-800 ng.mL for CLT and TEL respectively. The developed method is simple and rapid, analytical parameters were validated according to ICH guidelines and high sensitivity was achieved as represented by limits of detection (LOD) of 4.69 and 1.58 ng.mL for CLT and TEL respectively.
本研究首次开发并优化了一种同时测定人血浆样品中氯噻酮（CLT）和替米沙坦（TEL）以及它们新上市的药物片剂（Telmikind-CT 40®）中这两种药物的方法。该方法基于测量荧光强度，采用同步荧光模式结合三阶导数信号处理，使用 0.5%w/v 十六烷基三甲基溴化铵作为阳离子表面活性剂来增强荧光信号强度并提高方法灵敏度。CLT 和 TEL 的三阶导数同步光谱具有两个零交叉点，可分别在 362nm 和 351nm 处测定 CLT 和 TEL。仔细研究并优化了不同的实验参数，分别在 20-1200ng.mL 和 5-800ng.mL 浓度范围内为 CLT 和 TEL 构建了校准曲线。所开发的方法简单快速，分析参数根据 ICH 指南进行了验证，并且灵敏度很高，CLT 和 TEL 的检测限（LOD）分别为 4.69 和 1.58ng.mL。
