Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Horus University- Egypt, New Damietta, Egypt.
Sci Rep. 2023 Mar 2;13(1):3576. doi: 10.1038/s41598-023-30400-w.
Telmisartan (TEL) and Nebivolol (NEB) are frequently co-formulated in a single dosage form that is frequently prescribed for the treatment of hypertension, moreover, telmisartan is currently proposed to be used to treat COVID19-induced lung inflammation. Green rapid, simple, and sensitive synchronous spectrofluorimetric techniques for simultaneous estimation of TEL and NEB in their co-formulated pharmaceutical preparations and human plasma were developed and validated. Synchronous fluorescence intensity at 335 nm was used for TEL determination (Method I). For the mixture, the first derivative synchronous peak amplitudes (D) at 296.3 and 320.5 nm were used for simultaneous estimation of NEB and TEL, respectively (Method II). The calibration plots were rectilinear over the concentration ranges of 30-550 ng/mL, and 50-800 ng/mL for NEB and TEL, respectively. The high sensitivity of the developed methods allowed for their analysis in human plasma samples. NEB`s Quantum yield was estimated by applying the single-point method. The greenness of the proposed approaches was evaluated using the Eco-scale, National Environmental Method Index (NEMI), and Green Analytical Procedure Index (GAPI) methods.
替米沙坦（TEL）和比索洛尔（NEB）经常以单一剂量形式联合使用，用于治疗高血压，此外，替米沙坦目前被提议用于治疗 COVID19 引起的肺部炎症。开发并验证了用于同时测定其复方制剂和人血浆中替米沙坦和比索洛尔的快速、简单、灵敏的同步荧光分光光度法。在 335nm 处使用同步荧光强度来测定替米沙坦（方法 I）。对于混合物，在 296.3 和 320.5nm 处使用一阶导数同步峰幅度（D）分别用于同时测定比索洛尔和替米沙坦（方法 II）。校准曲线在替米沙坦和比索洛尔的浓度范围内分别为 30-550ng/mL 和 50-800ng/mL 呈线性。所开发方法的高灵敏度允许在人血浆样品中进行分析。通过应用单点法来估计 NEB 的量子产率。使用生态标度、国家环境方法指数（NEMI）和绿色分析程序指数（GAPI）方法评估所提出方法的绿色性。
