Hadley T J, Klotz F W, Pasvol G, Haynes J D, McGinniss M H, Okubo Y, Miller L H
Walter Reed Army Institute of Research, Washington, District of Columbia 20307-5100.
J Clin Invest. 1987 Oct;80(4):1190-3. doi: 10.1172/JCI113178.
To determine the ligands on erythrocytes for invasion by Plasmodium falciparum, we tested invasion into MkMk erythrocytes that lack glycophorins A and B and enzyme-treated erythrocytes by parasites that differ in their requirement for erythrocyte sialic acid. The 7G8 strain invaded MkMk erythrocytes and neuraminidase-treated normal erythrocytes with greater than 50% the efficiency of normal erythrocytes. In contrast, the Camp strain invaded MkMk erythrocytes at 20% of control and neuraminidase-treated normal erythrocytes at only 1.8% of control. Invasion of MkMk erythrocytes by 7G8 parasites was unaffected by treatment with neuraminidase but was markedly reduced by treatment with trypsin. In contrast, invasion of MkMk cells by Camp parasites was markedly reduced by neuraminidase but was unaffected by trypsin. We conclude that the 7G8 and Camp strains differ in ligand requirements for invasion and that 7G8 requires a trypsin sensitive ligand distinct from glycophorins A and B.
为了确定恶性疟原虫入侵红细胞的配体，我们用对红细胞唾液酸需求不同的疟原虫，测试了其对缺乏血型糖蛋白A和B的MkMk红细胞以及经酶处理的红细胞的入侵情况。7G8株入侵MkMk红细胞和神经氨酸酶处理的正常红细胞的效率，高于正常红细胞的50%。相比之下，Camp株入侵MkMk红细胞的效率为对照的20%，入侵神经氨酸酶处理的正常红细胞的效率仅为对照的1.8%。7G8疟原虫对MkMk红细胞的入侵不受神经氨酸酶处理的影响，但用胰蛋白酶处理后明显减少。相比之下，Camp疟原虫对MkMk细胞的入侵经神经氨酸酶处理后明显减少，但不受胰蛋白酶影响。我们得出结论，7G8和Camp株在入侵的配体需求上存在差异，并且7G8需要一种不同于血型糖蛋白A和B的对胰蛋白酶敏感的配体。
