Human Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, Hengyang Normal University, Hengyang 421008, China.
College of Life Science and Environment, Hengyang Normal University, Hengyang 421008, China.
Acta Biochim Biophys Sin (Shanghai). 2020 Dec 11;52(11):1274-1280. doi: 10.1093/abbs/gmaa107.
The venom of Lycosoidea spiders is a complex multicomponent mixture of neurotoxic peptides (main components) and antimicrobial peptides (AMPs) as minor components. In this study, we described the high-throughput identification and analysis of AMPs from Lycosa sinensis venom (named LS-AMPs) using a combination strategy that includes the following three different analysis approaches: (i) peptidomic analysis, namely reversed-phase high-performance liquid chromatography (RP-HPLC) separation plus top-down sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS); (ii) transcriptomic analysis, namely cDNA library construction plus DNA sequencing; (iii) bioinformatic analysis, namely analysis and prediction for molecular characters of LS-AMPs by the online biology databases. In total, 52 sequences of AMPs were identified from L. sinensis venom, and all AMPs can be categorized into eight different families according to phylogenetic analysis and sequence identity. This is the largest number of AMPs identified from a spider species so far. In the present study, we demonstrated molecular characteristics, such as complex precursor, N- and/or C-terminally truncated analogs, and C-terminal amidation of LS-AMPs from L. sinensis venom. This is a preliminary investigation on the molecular diversification of venom-derived AMPs from the wolf spider species (family Lycosidae), and a detailed investigation on the functional diversity of LS-AMPs will be preformed in the future.
狼蛛毒液是一种复杂的多组分混合物，由神经毒性肽（主要成分）和抗菌肽（AMPs）作为次要成分组成。在这项研究中，我们采用了一种组合策略，描述了从狼蛛毒液（命名为 LS-AMPs）中高通量鉴定和分析 AMPs 的方法，该策略包括以下三种不同的分析方法：（i）肽组学分析，即反相高效液相色谱（RP-HPLC）分离加基质辅助激光解吸/电离飞行时间质谱（MS）自上而下测序；（ii）转录组学分析，即 cDNA 文库构建加 DNA 测序；（iii）生物信息学分析，即通过在线生物学数据库对 LS-AMPs 的分子特征进行分析和预测。总共从狼蛛毒液中鉴定出 52 种 AMPs，所有 AMPs 根据系统发育分析和序列同一性可分为 8 个不同的家族。这是迄今为止从蜘蛛物种中鉴定出的最多的 AMPs 数量。在本研究中，我们展示了 LS-AMPs 的分子特征，如复杂的前体、N-和/或 C-末端截断类似物以及 C-末端酰胺化。这是对狼蛛科（Lycosidae）物种毒液来源的 AMPs 分子多样性的初步研究，未来将对 LS-AMPs 的功能多样性进行详细研究。
