Department of Thoracic Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Eur Rev Med Pharmacol Sci. 2020 Oct;24(19):9981-9989. doi: 10.26355/eurrev_202010_23211.
To explore the clinical significance of circRNF20 in non-small-cell lung carcinoma (NSCLC), and its regulatory effects on NSCLC cell functions by activating MAPK9.
Relative levels of circRNF20 and MAPK9 in NSCLC tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The relationship between circRNF20, MAPK9 and pathological factors in NSCLC patients was analyzed. Prognostic potentials of circRNF20 and MAPK9 in NSCLC were assessed by Kaplan-Meier method. The interaction between circRNF20 and MAPK9 was tested by Dual-Luciferase reporter assay. Regulatory effects of circRNF20 and MAPK9 on proliferative abilities in H358 and SPC-A1 cells were examined by Cell Counting Kit-8 (CCK-8) and colony formation assay.
CircRNF20 and MAPK9 were upregulated in NSCLC tissues than normal ones. They were correlated to T stage and poor prognosis in NSCLC patients, while their levels were unrelated to gender, age, and incidences of lymphatic and distant metastasis. Knockdown of circRNF20 attenuated proliferative abilities in H358 and SPC-A1 cells. On the contrary, the overexpression of MAPK9 yielded the opposite results. MAPK9 was the target gene binding circRNF20, which was able to reverse the regulatory effect of circRNF20 on NSCLC proliferation.
CircRNF20 and MAPK9 are upregulated in NSCLC cases, which are closely linked to T stage in NSCLC patients. They are independent prognostic factors for NSCLC. By activating MAPK9, circRNF20 stimulates NSCLC proliferation.
探讨 circRNF20 在非小细胞肺癌（NSCLC）中的临床意义及其通过激活 MAPK9 对 NSCLC 细胞功能的调节作用。
采用实时定量聚合酶链反应（qRT-PCR）检测 NSCLC 组织中 circRNF20 和 MAPK9 的相对水平。分析 circRNF20、MAPK9 与 NSCLC 患者病理因素的关系。采用 Kaplan-Meier 法评估 circRNF20 和 MAPK9 在 NSCLC 中的预后价值。采用双荧光素酶报告基因检测验证 circRNF20 与 MAPK9 的相互作用。通过细胞计数试剂盒-8（CCK-8）和集落形成实验检测 circRNF20 和 MAPK9 对 H358 和 SPC-A1 细胞增殖能力的调节作用。
circRNF20 和 MAPK9 在 NSCLC 组织中呈高表达，与 NSCLC 患者的 T 分期和不良预后相关，而与性别、年龄、淋巴和远处转移发生率无关。circRNF20 敲低可减弱 H358 和 SPC-A1 细胞的增殖能力，而过表达 MAPK9 则产生相反的结果。MAPK9 是 circRNF20 结合的靶基因，能够逆转 circRNF20 对 NSCLC 增殖的调节作用。
circRNF20 和 MAPK9 在 NSCLC 病例中呈高表达，与 NSCLC 患者的 T 分期密切相关。它们是非小细胞肺癌的独立预后因素。circRNF20 通过激活 MAPK9 促进 NSCLC 增殖。
