An Dexiang, Yang Jing, Ma Linli
Department of Ophthalmology, Lianyungang Maternal and Child Health Hospital, Lianyungang, Jiangsu Province, People's Republic of China.
Department of Pharmacy, Lianyungang Maternal and Child Health Hospital, Lianyungang, People's Republic of China.
Open Med (Wars). 2022 May 25;17(1):955-968. doi: 10.1515/med-2022-0483. eCollection 2022.
Circular RNAs (circRNAs) serve as essential players in diverse human cancers, including retinoblastoma (RB). In this study, the function of circRNA Ring Finger Protein 20 (circRNF20) in RB progression was investigated. Quantitative real-time polymerase chain reaction, western blot assay or immunohistochemistry assay was performed to determine the expression of circRNF20, miR-132-3p and Paired Box 6 (PAX6). Dual-luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay were utilized to verify the relationships among circRNF20, miR-132-3p and PAX6. experiment was done for circRNF20 function in tumor formation. It was found that ircRNF20 level was increased in RB tissues and linked to advanced tumor, nodes, metastases (TNM) stage and poor overall survival rate. Deficiency of circRNF20 suppressed cell proliferation, migration and invasion and induced apoptosis , as well as blocked tumor growth . circRNF20 directly targeted miR-132-3p and miR-132-3p overexpression inhibited RB cell progression. PAX6 was the target gene of miR-132-3p. Moreover, miR-132-3p inhibition or PAX6 overexpression reversed circRNF20 deficiency-mediated effects on RB cell malignant behaviors. In addition, exosomal circRNF20 was able to promote RB cell progression. Thus, we concluded that circRNF20 served as an oncogene in RB progression through the circRNF20/miR-132-3p/PAX6 pathway.
环状RNA（circRNAs）在包括视网膜母细胞瘤（RB）在内的多种人类癌症中发挥着重要作用。在本研究中，研究了环状RNA环指蛋白20（circRNF20）在RB进展中的功能。采用定量实时聚合酶链反应、蛋白质免疫印迹分析或免疫组织化学分析来确定circRNF20、miR-132-3p和配对盒6（PAX6）的表达。利用双荧光素酶报告基因检测、RNA免疫沉淀检测和RNA下拉检测来验证circRNF20、miR-132-3p和PAX6之间的关系。进行了circRNF20在肿瘤形成中功能的实验。研究发现，RB组织中circRNF20水平升高，且与肿瘤、淋巴结、转移（TNM）分期进展和总体生存率低相关。circRNF20缺失抑制细胞增殖、迁移和侵袭并诱导凋亡，以及阻断肿瘤生长。circRNF20直接靶向miR-132-3p，miR-132-3p过表达抑制RB细胞进展。PAX6是miR-132-3p的靶基因。此外，miR-132-3p抑制或PAX6过表达可逆转circRNF20缺失介导的对RB细胞恶性行为的影响。此外，外泌体circRNF20能够促进RB细胞进展。因此，我们得出结论，circRNF20通过circRNF20/miR-132-3p/PAX6途径在RB进展中作为癌基因发挥作用。
