Cassone A, De Bernardis F, Mondello F, Ceddia T, Agatensi L
Medical Mycology Unit, Istituto Superiore di Sanità, Rome, Italy.
J Infect Dis. 1987 Nov;156(5):777-83. doi: 10.1093/infdis/156.5.777.
Candida albicans isolates from nondiabetic, nonpregnant outpatients with vaginitis were compared for in vitro proteinase secretion with isolates from women without specific candidal vaginitis symptomatology (carriers). Proteinase production was assayed in medium containing bovine hemoglobin (BH-P; 39 isolates in 69 independent determinations) or bovine serum albumin (BSA-P; 39 isolates in a single determination each). All isolates had measurable, consistent BH-P secretion, and most also showed detectable BSA-P activity. However, isolates from patients were more proteolytic than those from carriers, with the difference being statistically highly significant. When the patients with vaginitis were categorized according to signs and symptoms, the highest BH-P values were recorded for those with full symptomatology, whereas the only BSA-P-negative isolates were from the group without vaginitis. Isolates from the patient and carrier groups did not differ as a whole in their growth potential in vitro, and all were germ tube responders in serum, independent of their source.
对从患有阴道炎的非糖尿病、非妊娠门诊患者中分离出的白色念珠菌与从无特定念珠菌性阴道炎症状的女性（携带者）中分离出的菌株进行体外蛋白酶分泌比较。在含有牛血红蛋白（BH-P；69次独立测定中的39株菌株）或牛血清白蛋白（BSA-P；每次单一测定中的39株菌株）的培养基中测定蛋白酶的产生。所有分离株均有可测量的、一致的BH-P分泌，且大多数也显示出可检测到的BSA-P活性。然而，来自患者的分离株比来自携带者的分离株蛋白水解能力更强，差异具有高度统计学意义。当根据体征和症状对阴道炎患者进行分类时，有完整症状的患者记录到最高的BH-P值，而唯一的BSA-P阴性分离株来自无阴道炎组。患者组和携带者组的分离株在体外的生长潜力总体上没有差异，并且所有菌株在血清中均为芽管反应者，与来源无关。
