从1型神经纤维瘤病患者中生成四条诱导多能干细胞系。
Generation of four iPSC lines from Neurofibromatosis Type 1 patients.
作者信息
Bozaoglu Kiymet, Shern Lee Wei, Haebich Kristina M, North Kathryn N, Payne Jonathan M, Lockhart Paul J
机构信息
Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.
Murdoch Children's Research Institute, Parkville, Australia; Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia.
出版信息
Stem Cell Res. 2020 Dec;49:102013. doi: 10.1016/j.scr.2020.102013. Epub 2020 Oct 1.
We describe the generation and characterisation of four human induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMC) from individuals with neurofibromatosis type (NF1). PBMC reprogramming was performed using a non-integrative Sendai virus containing the reprogramming factors OCT4, SOX2, MYC and KLF4. All iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry (EPCAM, TRA-1-81, SSEA1 and CD9) and immunofluorescence (OCT4 and Nanog). Differentiation of the cells into the three embryonic germ layers was confirmed using immunofluorescence. These iPSC lines are a valuable pre-clinical resource to study the molecular mechanisms underlying NF1.
我们描述了从患有1型神经纤维瘤病（NF1）个体的外周血单个核细胞（PBMC）中生成并鉴定四种人类诱导多能干细胞（iPSC）系的过程。使用含有重编程因子OCT4、SOX2、MYC和KLF4的非整合仙台病毒进行PBMC重编程。所有iPSC系均表现出正常核型，并通过流式细胞术（EPCAM、TRA-1-81、SSEA1和CD9）和免疫荧光（OCT4和Nanog）验证了多能性。使用免疫荧光确认了细胞向三个胚胎胚层的分化。这些iPSC系是研究NF1潜在分子机制的宝贵临床前资源。
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