Matsuyama S, Mizuno T, Mizushima S
J Bacteriol. 1986 Dec;168(3):1309-14. doi: 10.1128/jb.168.3.1309-1314.1986.
The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mutation was isolated. The suppressor mutation also suppressed the LamB- PhoA- phenotype caused by the envZ11 mutation. The mutation occurred in the ompR gene and hence was termed ompR77. The ompR77 mutation alone produced no obvious phenotype. Functioning of the ompR77 allele remained envZ gene dependent. Although the ompR77 mutation suppressed the envZ11 mutation, it did not suppress a mutation that occurred in another position within the envZ gene (envZ160). These results indicate that OmpR and EnvZ, two regulatory proteins, functionally interact with each other.
ompR和envZ基因共同构成ompB操纵子，参与大肠杆菌主要外膜蛋白OmpF和OmpC蛋白的渗透压调节表达。envZ11突变导致OmpF - OmpC组成型表型。分离出一个抑制envZ11突变引起的缺陷的突变体。该抑制突变也抑制了由envZ11突变引起的LamB - PhoA表型。该突变发生在ompR基因中，因此被称为ompR77。单独的ompR77突变不产生明显的表型。ompR77等位基因的功能仍然依赖于envZ基因。虽然ompR77突变抑制了envZ11突变，但它没有抑制envZ基因内另一个位置发生的突变（envZ160）。这些结果表明，两种调节蛋白OmpR和EnvZ在功能上相互作用。
