Slauch J M, Garrett S, Jackson D E, Silhavy T J
Department of Molecular Biology, Princeton University, New Jersey 08544.
J Bacteriol. 1988 Jan;170(1):439-41. doi: 10.1128/jb.170.1.439-441.1988.
The regulatory proteins OmpR and EnvZ are both required to activate expression of the genes for the major outer membrane porin proteins, OmpF and OmpC, of Escherichia coli K-12. Here we show that OmpR, under certain conditions, could activate porin expression in the complete absence of EnvZ. In addition, the pleiotropic phenotypes conferred by a particular envZ mutation (envZ473) required the presence of functional OmpR protein. These results lead us to conclude that EnvZ and OmpR act in sequential fashion to activate porin gene expression; i.e., EnvZ modifies or in some way directs OmpR, which in turn acts at the appropriate porin gene promoter.
调节蛋白OmpR和EnvZ都是激活大肠杆菌K - 12主要外膜孔蛋白OmpF和OmpC基因表达所必需的。在这里我们表明，在某些条件下，OmpR在完全没有EnvZ的情况下也能激活孔蛋白的表达。此外，特定envZ突变（envZ473）赋予的多效性表型需要功能性OmpR蛋白的存在。这些结果使我们得出结论，EnvZ和OmpR以顺序方式作用来激活孔蛋白基因表达；即EnvZ修饰或以某种方式指导OmpR，而OmpR反过来作用于适当的孔蛋白基因启动子。
