Department of Hepatobiliary Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China (mainland).
Department of Hepatopancreatobiliary Surgery, People's Hospital of Deyang City, Deyang, Sichuan, China (mainland).
Med Sci Monit. 2020 Oct 23;26:e925727. doi: 10.12659/MSM.925727.
BACKGROUND Acute pancreatitis (AP) is generally a self-limiting inflammatory disease, but is associated with a high mortality rate when severe. The present study aimed to investigate the effects of rhein and honokiol on AP. MATERIAL AND METHODS Thirty mice were randomly divided into 5 groups (n=6 per group): blank control, AP model, AP+rhein, AP+honokiol, and AP+rhein+honokiol. The AP model was prepared by intraperitoneal injection of cerulein and lipopolysaccharide (LPS). We observed the pathological changes of the pancreas by hematoxylin and eosin (H&E) staining. A mouse amylase kit was utilized to detect the level of amylase content in serum. Gas chromatography mass spectrometer analysis was performed to detect the differences in metabolites among the blank control, AP model, and AP+rhein+honokiol groups. RESULTS The serum amylase level was significantly higher in the AP model, which suggested that the AP model was constructed successfully. The AP+rhein+honokiol group had significantly reduced interstitial edema, inflammatory cell infiltration, hemorrhage, and necrosis. In addition, the rhein and honokiol treatment influenced some of the metabolic pathways in AP, including riboflavin metabolism, glycerophospholipid metabolism, linoleic acid metabolism, and the pentose and glucuronate interconversions pathway. CONCLUSIONS This study showed that the combination of rhein and honokiol ameliorated pathological changes in the pancreas of mice with AP.
急性胰腺炎（AP）通常是一种自限性炎症性疾病，但当病情严重时，其死亡率很高。本研究旨在探讨大黄酸和厚朴酚对 AP 的影响。
30 只小鼠随机分为 5 组（每组 6 只）：空白对照组、AP 模型组、AP+大黄酸组、AP+厚朴酚组和 AP+大黄酸+厚朴酚组。通过腹腔注射鹅脱氧胆酸钠和脂多糖（LPS）制备 AP 模型。通过苏木精-伊红（H&E）染色观察胰腺的病理变化。采用小鼠淀粉酶试剂盒检测血清中淀粉酶含量。采用气相色谱-质谱联用分析检测空白对照组、AP 模型组和 AP+大黄酸+厚朴酚组之间的代谢物差异。
AP 模型组血清淀粉酶水平显著升高，表明 AP 模型构建成功。AP+大黄酸+厚朴酚组间质水肿、炎症细胞浸润、出血和坏死明显减轻。此外，大黄酸和厚朴酚的治疗影响了 AP 中的一些代谢途径，包括核黄素代谢、甘油磷脂代谢、亚油酸代谢和戊糖和葡萄糖醛酸相互转化途径。
本研究表明，大黄酸和厚朴酚的联合应用改善了 AP 小鼠胰腺的病理变化。
