Instituto Politécnico Nacional, Centro de Biotecnología Genómica, Laboratorio de Biomedicina Molecular, Blvd. del Maestro esquina Elías Piña s/n, Colonia Narciso Mendoza, 88710, Cd. Reynosa, Tamaulipas, México.
Animal and Plant Health Agency, Virology Department, Rabies and Viral Zoonoses, Woodham Lane Addlestone, Surrey, KT15 3NB, United Kingdom.
Acta Trop. 2021 Jan;213:105730. doi: 10.1016/j.actatropica.2020.105730. Epub 2020 Oct 21.
Mosquitoes are commonly identified to species level using morphological traits, but complementary methods for identification are often necessary when specimens are collected as immature stages, stored inadequately, or when delineation of species complexes is problematic. DNA-barcoding using the mitochondrial cytochrome c oxidase subunit 1 (COI) gene is one such tool used for the morphological identification of species. A comprehensive entomological survey of mosquito species in Mexico State identified by COI DNA barcoding and morphology is documented in this paper. Specimens were collected from all the physiographic provinces in Mexico State between 2017 and 2019. Overall, 2,218 specimens were collected from 157 localities representing both subfamilies Anophelinae and Culicinae. A species checklist that consists of 6 tribes, 10 genera, 20 subgenera, and 51 species, 35 of which are new records for Mexico State, is provided. Three hundred and forty-two COI sequences of 46 species were analysed. Mean intraspecific and interspecific distances ranged between 0% to 3.9% and from 1.2% to 25.3%, respectively. All species groups were supported by high bootstraps values in a Neighbour-Joining analysis, and new COI sequences were generated for eight species: Aedes chionotum Zavortink, Ae. vargasi Schick, Ae. gabriel Schick, Ae. guerrero Berlin, Ae. ramirezi Vargas and Downs, Haemagogus mesodentatus Komp and Kumm, Culex restrictor Dyar and Knab, and Uranotaenia geometrica Theobald. This study provides a detailed inventory of the Culicidae from Mexico State and discusses the utility of DNA barcoding as a complementary tool for accurate mosquito species identification in Mexico.
蚊子通常通过形态特征鉴定到种的水平，但当标本作为未成熟阶段采集、储存不当或物种复合体的划分存在问题时，通常需要补充鉴定方法。使用线粒体细胞色素 c 氧化酶亚基 1（COI）基因进行 DNA 条形码是用于物种形态鉴定的一种此类工具。本文记录了使用 COI DNA 条形码和形态学对墨西哥州蚊子物种的全面昆虫学调查结果。这些标本是在 2017 年至 2019 年期间从墨西哥州所有地貌省份采集的。总共从代表 Anophelinae 和 Culicinae 两个亚科的 157 个地点收集了 2,218 个标本。提供了一个包含 6 个部落、10 个属、20 个亚属和 51 个物种的物种清单，其中 35 个是墨西哥州的新记录。分析了 46 个物种的 342 个 COI 序列。种内和种间平均距离分别在 0%到 3.9%和 1.2%到 25.3%之间。在邻接法分析中，所有物种组都得到了高置信度支持，并且为 8 个物种生成了新的 COI 序列：Aedes chionotum Zavortink、Ae. vargasi Schick、Ae. gabriel Schick、Ae. guerrero Berlin、Ae. ramirezi Vargas and Downs、Haemagogus mesodentatus Komp and Kumm、Culex restrictor Dyar and Knab 和 Uranotaenia geometrica Theobald。本研究提供了墨西哥州库蚊科的详细清单，并讨论了 DNA 条形码作为一种补充工具在墨西哥准确鉴定蚊子物种的实用性。
