J Am Mosq Control Assoc. 2021 Dec 1;37(4):198-207. doi: 10.2987/21-6967.
Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages and large numbers of some species that are members of morphologically similar species complexes. In the present study, the mosquitoes collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged 1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable approach to support mosquito species identification in Mexico.
准确识别蚊子种类对于支持涉及分布和蚊子控制的研究项目至关重要。由于不同生命阶段的形态相似性以及某些物种数量庞大，属于形态相似的物种复合体，因此仅凭形态特征很难识别许多蚊子种类。在本研究中，使用形态学和分子方法（线粒体细胞色素 c 氧化酶亚基 I [COI] DNA 条码）对在墨西哥东南部潘塔努斯生物圈保护区采集的蚊子进行了评估。共采集到 10 属 35 种 1576 只标本，主要为成蚊。共分析了 225 个 COI DNA 条码序列；大多数物种在邻接法、最大似然法和贝叶斯推断树中形成了支持良好的群体。种内 Kimura 2-参数（K2P）遗传距离平均为 1.52%。在 Anopheles crucians s.l. 中观察到 6.20%的种内 K2P 距离，而在 Culex erraticus 和 Cx. conspirator 中则存在深度分裂。本研究表明，COI DNA 条码为支持墨西哥蚊子种类鉴定提供了一种可靠的方法。
