Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
J Hazard Mater. 2021 Mar 5;405:124259. doi: 10.1016/j.jhazmat.2020.124259. Epub 2020 Oct 13.
There is a continuing high demand to design effective sensors for the determination of heavy metal ions (HMIs) since they are hazardous to both human health and the environment. In this study, we reported a facile fluorescent sensor array for rapid discrimination of HMIs based on a single gold nanocluster (AuNC) probe. This AuNC probe was prepared by using 2-mercapto-1-methylimidazole (MMI) as a ligand and polyvinypyrrolidone (PVP) as a dispersing agent. The fluorescence emission of PVP/MMI-AuNC was observed to be closely related to the pH value of the aqueous solution, which displays yellow (λ = 512 nm) and red (λ = 700 nm) fluorescence at pH 12.0 and 6.0, respectively. Further experiments indicated that different HMIs can produce differential effects on the photoluminescence of PVP/MMI-AuNC and thus generate distinct fluorescent responses at 512 and 700 nm. On the basis of this phenomenon, a fluorescent sensor array based on the PVP/MMI-AuNC was then built by simply changing pH value in the sensor element. A total of seven HMIs had their unique response patterns and were successfully distinguished by hierarchical cluster analysis and linear discriminant analysis both in buffer solution and spiked water samples, achieving 100% identification accuracy. This study provides a simple and powerful fingerprinting sensing platform for multiple HMIs, showing broad application prospects in the field of environmental monitoring.
由于重金属离子（HMIs）对人类健康和环境都具有危害性，因此设计有效的传感器来测定 HMIs 的需求持续高涨。在本研究中，我们报道了一种基于单个金纳米簇（AuNC）探针的快速区分 HMIs 的简便荧光传感器阵列。该 AuNC 探针是通过使用 2-巯基-1-甲基咪唑（MMI）作为配体和聚乙烯吡咯烷酮（PVP）作为分散剂制备的。PVP/MMI-AuNC 的荧光发射与水溶液的 pH 值密切相关，在 pH 值为 12.0 和 6.0 时分别显示出黄色（λ = 512nm）和红色（λ = 700nm）荧光。进一步的实验表明，不同的 HMIs 可以对 PVP/MMI-AuNC 的光致发光产生不同的影响，从而在 512nm 和 700nm 处产生不同的荧光响应。基于这一现象，我们通过简单地改变传感器元件中的 pH 值，构建了基于 PVP/MMI-AuNC 的荧光传感器阵列。在缓冲溶液和加标水样中，七种 HMIs 都具有独特的响应模式，并通过层次聚类分析和线性判别分析成功区分，识别准确率达到 100%。本研究为多种 HMIs 提供了一种简单而强大的指纹传感平台，在环境监测领域具有广阔的应用前景。
