Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia.
Department of Therapy, Geriatrics, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research and Clinical Center of Federal Medical-Biological Agency of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2020 Oct;169(6):821-824. doi: 10.1007/s10517-020-04986-4. Epub 2020 Oct 24.
Verification of signaling molecules in the saliva is a non-invasive method of diagnosis and evaluation of treatment effectiveness in different pathologies. Sirtuins (SIRT), proteins from NAD-dependent histone deacetylases, are supposed to be involved in the pathogenesis of Alzheimerэs disease. Age-related decrease in sirtuins expression induces many pathophysiological processes that could lead to neurodegeneration. We studied the expression of SIRT1, SIRT3, SIRT5, and SIRT6 in the hippocampus and saliva of humans without neurological pathologies and in patients with Alzheimer's disease of elderly and senile age. In elderly and senile patients, the expression of SIRT1, SIRT3, and SIRT6 in the hippocampus and saliva was 1.5-4.9-fold reduced in comparison with healthy individuals of the corresponding age. In healthy senile persons, the expression of SIRT6 in the hippocampus and saliva was 2.5-4.5-fold lower than in healthy elderly individuals. Measurement of SIRT1, SIRT3, and SIRT6 concentration in the saliva can be used as an additional method for intravital non-invasive diagnosis of Alzheimer's disease in patients of advanced age. SIRT6 concentration in the saliva can be recommended as a marker for assessment of the rate of aging.
唾液中信号分子的验证是一种非侵入性的诊断方法，可用于评估不同病理情况下的治疗效果。Sirtuins（SIRT）是 NAD 依赖性组蛋白去乙酰化酶的蛋白质，被认为与阿尔茨海默病的发病机制有关。与年龄相关的 Sirtuins 表达减少会引起许多病理生理过程，从而导致神经退行性变。我们研究了 SIRT1、SIRT3、SIRT5 和 SIRT6 在无神经病理的人类海马体和唾液中的表达，以及在老年和老年期阿尔茨海默病患者中的表达。在老年和老年患者中，SIRT1、SIRT3 和 SIRT6 在海马体和唾液中的表达与相应年龄的健康个体相比降低了 1.5-4.9 倍。在健康的老年人群中，SIRT6 在海马体和唾液中的表达比健康的老年人群低 2.5-4.5 倍。唾液中 SIRT1、SIRT3 和 SIRT6 浓度的测量可作为对高龄患者进行活体非侵入性阿尔茨海默病诊断的附加方法。唾液中 SIRT6 的浓度可作为评估衰老速度的标志物。
