State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China.
Bioresour Technol. 2020 Dec;318:124268. doi: 10.1016/j.biortech.2020.124268. Epub 2020 Oct 17.
This study aims to develop a spore fermentation method instead of fruiting body extraction for the production of Tremella aurantialba polysaccharide (TAPS). The purified spore strain Tremella aurantialba NX-20 was isolated for TAPS fermentation. The fermented TAPS and the extracted TAPS had the same monosaccharide composition but higher molecular weight. The conditions for TAPS fermentation by NX-20 were optimized, which were 25 °C, pH 7, 10% initial inoculum and 5 days. To further reduce the processing steps and costs of the medium, tofu wastewater (TW) was used to replace defatted soybean meal (DSM). In a 7.5 L fermentation tank, 40 g/L glucose with 10-fold diluted TW was used as the medium. For TAPS fermentation, a maximum yield of 15.02 ± 0.40 g/L was achieved. In conclusion, this study provides a feasible strategy for the efficient preparation of TAPS through spore fermentation.
本研究旨在开发一种以孢子发酵代替子实体提取来生产银耳多糖（TAPS）的方法。从银耳菌株中分离出纯孢子菌株银耳 NX-20 用于 TAPS 发酵。发酵的 TAPS 和提取的 TAPS 具有相同的单糖组成，但分子量更高。优化了 NX-20 发酵 TAPS 的条件，即 25°C、pH7、初始接种量 10%和发酵 5 天。为了进一步减少培养基的加工步骤和成本，使用豆腐废水（TW）替代脱脂大豆粉（DSM）。在 7.5 L 发酵罐中，以 40 g/L 葡萄糖和 10 倍稀释的 TW 为培养基进行 TAPS 发酵，最高产量达到 15.02±0.40 g/L。总之，本研究为通过孢子发酵高效制备 TAPS 提供了一种可行的策略。
