Department of Physical Education, Chengdu University of Information Technology, Chengdu 611130, China.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Molecules. 2023 Jul 19;28(14):5498. doi: 10.3390/molecules28145498.
Polygonati Rhizoma is a widely used traditional Chinese medicine (TCM) with complex pre-processing steps. Fermentation is a common method for processing TCM to reduce herb toxicity and enhance their properties and/or produce new effects. Here, in this study, using and , we aimed to evaluate the potential application of solid fermentation in isolating different functional polysaccharides from Hua. With hot water extraction, ethanol precipitation, DEAE anion exchange chromatography and gel filtration, multiple neutral and acidic polysaccharides were obtained, showing different yields, content, compositions and functional groups after fermentation. Combining in vitro experiments and in vivo aging and immunosuppressed mouse models, we further compared the antioxidant and immunomodulating bioactivities of these polysaccharides and found a prominent role of a natural polysaccharide (BNP) from fermented via in regulating intestinal antioxidant defense and immune function, which may be a consequence of the ability of BNP to modulate the homeostasis of gut microbiota. Thus, this work provides evidence for the further development and utilization of with fermentation, and reveals the potential values of BNP in the treatment of intestinal disorders.
黄精是一种广泛使用的中药，具有复杂的预处理步骤。发酵是一种常见的中药加工方法，可降低草药毒性，增强其特性和/或产生新的效果。在这里，在这项研究中，我们使用 和 ，旨在评估固体发酵在从黄精中分离不同功能多糖方面的潜在应用。通过热水提取、乙醇沉淀、DEAE 阴离子交换色谱和凝胶过滤，获得了多种中性和酸性多糖，它们在发酵后的产率、含量、组成和功能基团方面表现出不同的特性。结合体外实验和体内衰老及免疫抑制小鼠模型，我们进一步比较了这些多糖的抗氧化和免疫调节生物活性，发现发酵黄精天然多糖（BNP）通过 调节肠道抗氧化防御和免疫功能的作用显著，这可能是 BNP 调节肠道微生物组平衡的能力的结果。因此，这项工作为发酵黄精的进一步开发和利用提供了证据，并揭示了 BNP 在治疗肠道疾病方面的潜在价值。
