Fieldes Mathieu, Ahmed Engi, Bourguignon Chloé, Mianné Joffrey, Martin Mélanie, Arnould Cécile, Vachier Isabelle, Assou Said, De Vos John, Bourdin Arnaud
IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France.
IRMB, Univ Montpellier, INSERM, CHU Montpellier, Montpellier 34000, France; Department of Respiratory Diseases, CHU Montpellier, Arnaud de Villeneuve Hospital, INSERM, Montpellier 34000, France.
Stem Cell Res. 2020 Dec;49:102037. doi: 10.1016/j.scr.2020.102037. Epub 2020 Oct 8.
Human pluripotent stem cells (hiPSC) are highly valuable tools to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized 40-year-old healthy male nonsmoking donor. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai Virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi002-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.
人多能干细胞（hiPSC）是模拟肺发育和慢性支气管疾病的极有价值的工具。我们从一名特征明确的40岁健康男性非吸烟供体中生成了一个hiPSC系。使用无整合仙台病毒对外周血单个核细胞（PBMC）进行重编程。该细胞系具有正常的核型，表达多能性标志，并分化为三个原始胚层。报道的UHOMi002 - A iPSC系可作为对照用于模拟肺发育、研究人类慢性支气管疾病和药物测试。
