Université de Paris, Imagine Institute, iPSC Core Facility, INSERM UMR U1163, F-75015 Paris, France.
Université de Paris, Imagine Institute, iPSC Core Facility, INSERM UMR U1163, F-75015 Paris, France.
Stem Cell Res. 2022 May;61:102755. doi: 10.1016/j.scr.2022.102755. Epub 2022 Mar 21.
Human pluripotent stem cells are a powerful tool to study development, to model diseases or as cellular substrates for drug screening. We generated a human induced pluripotent stem cell (hiPSC) line from a healthy control donor. Peripheral blood mononuclear cells (PBMCs) from this donor were reprogrammed using integration-free Sendai virus. This cell line had normal karyotype, expressed pluripotency hallmarks and differentiated into the three primary germ layers.
人多能干细胞是研究发育、疾病建模或作为药物筛选的细胞底物的有力工具。我们从健康对照供体中生成了一个人诱导多能干细胞（hiPSC）系。使用无整合的 Sendai 病毒对该供体的外周血单核细胞（PBMC）进行重编程。该细胞系具有正常核型，表达多能性标志物，并分化为三个初级胚层。
