Shafik Nevine F, El Ashry Mona S, Badawy Ragia H, Hussein Marwa M, Hassan Naglaa M
Department of Clinical Pathology, National Cancer Institute (NCI), Cairo University, Fom Elkhalig, Kasr elaini street, Cairo, 11796 Egypt.
Department of Medical Oncology, National Cancer Institute (NCI), Cairo University, Cairo, 11796 Egypt.
Indian J Hematol Blood Transfus. 2020 Oct;36(4):652-660. doi: 10.1007/s12288-020-01278-9. Epub 2020 Mar 30.
The aim of this work is to investigate the different expression patterns of B cell-specifics moloney murine Leukemia virus integration site-1 (BMI-1) and brain and acute leukemia, cytoplasmic (BAALC) genes, their prognostic and clinical significance in newly diagnosed cytogenetically heterogenous adult acute myeloid leukemia patients. BMI-1 and BAALC expression was detected in the bone marrow of patients using quantitative real-time reverse transcription polymerase chain reaction with cut off value set at 50th percentile for both genes. BMI-1 and BAALC overexpression was detected in 50% of cases which suggest their potential as molecular markers. A statistical significant correlation was found between BMI-1 expression with hepatomegaly ( value = 0.007), hemoglobin level-grouped ( value = 0.047) and cytogenetic risk groups ( value = 0.036). There was a statistically significant correlation between BAALC and age ( value = 0.015), lymphadenopathy ( value = 0.043), CD34 expression ( value = 0.003) and near statistical significance with FAB sub-groups ( value = 0.054). No statistical significance was noted for other hematological findings and response to treatment except for gene and treatment response ( value = 0.014). No statistical significance in overall survival and disease free survival for both genes was found. Their prospective screening in combination with other molecular markers can help refine myeloid leukemia staging and prognosis toward optimizing therapeutic interventions.
这项工作的目的是研究B细胞特异性莫洛尼鼠白血病病毒整合位点-1（BMI-1）和脑与急性白血病细胞质（BAALC）基因的不同表达模式，以及它们在新诊断的细胞遗传学异质性成年急性髓系白血病患者中的预后和临床意义。使用定量实时逆转录聚合酶链反应在患者骨髓中检测BMI-1和BAALC表达，两个基因的临界值均设定为第50百分位数。在50%的病例中检测到BMI-1和BAALC过表达，这表明它们作为分子标志物的潜力。发现BMI-1表达与肝肿大（P值 = 0.007）、血红蛋白水平分组（P值 = 0.047）和细胞遗传学风险组（P值 = 0.036）之间存在统计学显著相关性。BAALC与年龄（P值 = 0.015）、淋巴结病（P值 = 0.043）、CD34表达（P值 = 0.003）之间存在统计学显著相关性，与FAB亚组接近统计学显著相关性（P值 = 0.054）。除了 基因与治疗反应（P值 = 0.014）外，其他血液学检查结果和治疗反应未发现统计学意义。两个基因在总生存期和无病生存期方面均未发现统计学意义。将它们与其他分子标志物联合进行前瞻性筛查有助于优化髓系白血病分期和预后，以优化治疗干预措施。
